Tail Coiling Assay in Zebrafish (Danio rerio) Embr... - BV FAPESP
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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Tail Coiling Assay in Zebrafish (Danio rerio) Embryos: Stage of Development, Promising Positive Control Candidates, and Selection of an Appropriate Organic Solvent for Screening of Developmental Neurotoxicity (DNT)

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Author(s):
de Oliveira, Andreia A. S. [1] ; Brigante, Tamires A. V. [2] ; Oliveira, Danielle P. [1, 3]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, BR-14040903 Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Pharmacol, BR-14049900 Ribeirao Preto - Brazil
[3] Natl Inst Alternat Technol Detect, Toxicol Evaluat & Removal Micropollutants & Radio, BR-14800060 Araraquara, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: WATER; v. 13, n. 2 JAN 2021.
Web of Science Citations: 0
Abstract

It is relatively recent that tail coiling assay in zebrafish (Danio rerio) embryos has been proposed as an alternative method to screen for developmental neurotoxicity (DNT) induced by chemicals. Despite the considerable use of the method, there is no consensus related to the most suitable age of embryos and other experimental parameters. Non-exposed embryos were videotaped for tail-coiling activity from 18 to 54 h post-fertilization (hpf) and after exposure to positive control candidates (caffeine, fluoxetine, and tricaine (MS-222)) and organic solvents (acetone, dimethyl-sulfoxide (DMSO), and ethanol) from 26.0 to 28.5 hpf. Results demonstrated that embryos from 22 to 29 hpf presented a constant coiling activity, with no significant differences between the activity measurements. We also found that stimulant properties of caffeine and the anesthetic effects of MS-222 induced hyperactivity and hypoactivity, respectively. Finally, even using DMSO at 1%, it seems to be safer as a solvent for neurotoxicity evaluation by tail coiling assay. The period from 26.0 to 28.5 hpf was appropriate for a fast protocol of tail coiling assay. Caffeine and MS-222 were demonstrated to be promising positive control candidates, whereas DMSO was considered the most appropriate solvent choice for tail coiling assay. (AU)

FAPESP's process: 14/50945-4 - INCT 2014: National Institute for Alternative Technologies of Detection, Toxicological Evaluation and Removal of Micropollutants and Radioactivies
Grantee:Maria Valnice Boldrin
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 18/13249-0 - Comparative toxicity analysis of trifluralin and tebuthiuron herbicides and its commercial formulations: effects on embryo-larval developmental of Zebrafish (Danio rerio)
Grantee:Andréia Ávila Soares de Oliveira
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 18/24298-2 - USE OF MULTI-BIOMARkER STRATEGY IN ZEBRAFISH AS A PLATFORM TO ESTIMATE NEUROCOMPORTAMENTAL EFFECTS INDUCED BY FLAME RETARDANTS
Grantee:Danielle Palma de Oliveira
Support Opportunities: Regular Research Grants