ELOVL5 Participates in Embryonic Lipid Determination of Cellular Membranes and Cytoplasmic Droplets

Author(s)

Lanzarini, Franciele ^[1] ; Pereira, Fernanda Alves ^[1] ; Camargo, Janine de ^{[1, 2]} ; Oliveira, Andressa Minozzo ^[1] ; Belaz, Katia Roberta Anacleto ^{[3, 4]} ; Melendez-Perez, Jose Javier ^[5] ; Eberlin, Marcos Nogueira ^[6] ; Brum, Mario Celso Sperotto ^[1] ; Mesquita, Fernando Silveira ^[1] ; Sudano, Mateus Jose ^{[1, 2, 7]}

Affiliation

^[1] Univ Fed Pampa, Curso Med Vet, BR-96460000 Uruguaiana, RS - Brazil

^[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, UFSCar, BR-13565905 Sao Carlos, SP - Brazil

^[3] Univ Estadual Campinas, Inst Quim, UNICAMP, BR-13083970 Campinas, SP - Brazil

^[4] Univ Fed Catalao, Inst Quim, UFG UFCAT, BR-75705220 Catalao, Go - Brazil

^[5] Unidade Integrada Farmacol & Gastroenterol, BR-12916900 Braganca Paulista, SP - Brazil

^[6] Univ Presbiteriana Mackenzie, Nucleo Pesquisa Mackenzie Ciencia Fe & Soc, BR-01302907 Sao Paulo, SP - Brazil

^[7] Univ Fed ABC, Ctr Ciencias Nat & Humana, BR-09210580 Santo Andre, SP - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 3 FEB 2021.
• Web of Science Citations: 0

Abstract

Embryonic lipids are crucial for the formation of cellular membranes and dynamically participate in metabolic pathways. Cells can synthesize simple fatty acids, and the elongation of fatty acids facilitates the formation of complex lipids. The aim of this work was to investigate the involvement of the elongation of very long chain fatty acid enzyme 5 (ELOVL5) in embryonic development and lipid determination. Bovine embryos were produced in vitro using a standard protocol and randomly divided to receive one of three treatments at Day 4: morpholino (Mo) gene expression knockdown assay for ELOVL5 (ELOVL5-Mo), Mo antisense oligonucleotides for the thalassemic beta-globulin human mRNA (technical control Mo), and placebo (biological control). The phenotypes of embryonic development, cell number, ELOVL5 protein abundance, lipid droplet deposits, and lipid fingerprint were investigated. No detrimental effects (p > 0.05) were observed on embryo development in terms of cleavage (59.4 +/- 3.5%, 63.6 +/- 4.1%, and 65.4 +/- 2.2%), blastocyst production (31.3 +/- 4.2%, 28.1 +/- 4.9%, and 36.1 +/- 2.1%), and blastocyst cell number (99.6 +/- 7.7, 100.2 +/- 6.2, 86.8 +/- 5.6), respectively, for biological control, technical control Mo, and ELOVL5-Mo. ELOVL5 protein abundance and cytoplasmic lipid droplet deposition were increased (p < 0.05) in ELOVL5-Mo-derived blastocysts compared with the controls. However, seven lipid species, including phosphatidylcholines, phosphatidylethanolamines, and triacylglycerol, were downregulated in the ELOVL5-Mo-derived blastocysts compared with the biological control. Therefore, ELOVL5 is involved in the determination of embryonic lipid content and composition. Transient translational blockage of ELOVL5 reduced the expression of specific lipid species and promoted increased cytoplasmic lipid droplet deposition, but with no apparent deleterious effect on embryonic development and blastocyst cell number. (AU)

• FAPESP's process: 12/07206-0 - Search for a Molecular Signature of Receptivity in Cattle by Mass Spectrometry
• Grantee: Katia Roberta Anacleto Belaz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral