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Lipids profile changes associated to pluripotency regulatory mechanisms during mesenchymal cells reprogramming to human induced pluripotent stem cells (IPS)

Grant number: 12/01394-0
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2012
Effective date (End): January 09, 2016
Field of knowledge:Biological Sciences - Morphology - Embryology
Principal Investigator:Flávio Vieira Meirelles
Grantee:Pedro Ratto Lisboa Pires
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated scholarship(s):14/05405-1 - Development and validation of Multiplexed bovine plasma cytokine measurement using multiple reaction monitoring (MRM) mass spectrometry, BE.EP.DR

Abstract

Generation of induced pluripotent stem cells (iPS) from somatic cells has shown that adult mammalian cells can be reprogramed to a pluripotent condition through the implantation of some embryonic transcription factors. This discovery has raised fundamental matters about the mechanisms that, through this transcription factors, influenced cells epigenetically and its differentiation potential, during reprograming and normal development. Lipids and lipoprotein components affect some aspects of cell behavior during its differentiation and survival. This components act mainly through the activation of membrane receptors, responsible for activating cytoplasmic regulatory factors and stimulating or blocking specific signals of transduction pathways. Specific chromatin modulations and the activity of transcriptional factors are directly influenced by these signals (Chawla et al., 2001; Stulnig et al., 2003; Rawson et al., 2003). Hence, specific study about cellular and molecular mechanisms involved on reprograming became essential for major understanding of this process, thus, are necessary for better characterization and efficiency. Therefore, this study's proposal is the characterization of iPS cells from human mesenchymal cells and the investigation of the genetic induction at the lipidic level (lipidic profile). To do so, mRNA frequency of genes known as essential to pluripotency maintenance will be analyzed, as well as the definition of the lipidic profile during cells pluripotency induction process and differentiation process, after colonies were obtained.