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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Progesterone dose during synchronization treatment alters luteinizing hormone receptor and steroidogenic enzyme mRNA abundances in granulosa cells of Nellore heifers

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Author(s):
Dias, H. P. [1] ; Poole, R. K. [2] ; Albuquerque, J. P. [1] ; dos Santos, P. H. [3] ; Castilho, A. C. S. [4] ; Pohler, K. G. [2] ; Vasconcelos, J. L. M. [1]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ, Dept Anim Prod, BR-18168000 Botucatu, SP - Brazil
[2] Texas A&M Univ, Dept Anim Sci, Pregnancy & Dev Programming Area Excellence, College Stn, TX 77843 - USA
[3] Sao Paulo State Univ, Inst Biosci, BR-18168000 Botucatu, SP - Brazil
[4] Univ Western Sao Paulo, Presidente Prudente - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Animal Reproduction Science; v. 225, FEB 2021.
Web of Science Citations: 0
Abstract

The objective was to investigate effects of progesterone (P-4) dose on abundance of luteinizing hormone receptor (LHCGR), aromatase (CYP19A1), 3 beta-hydroxysteroid dehydrogenase (HSD3B1), and other steroidogenic mRNA transcripts in granulosa cells from dominant follicles. Nellore heifers were assigned to one of six groups: new, first-use controlled internal drug release device (CIDR1) inserted for 5 days (Large-P-4-dose-D5; n = 7) or 6 days (Large-P-4-dose-D6; n = 8), prostaglandin (PG)F-2 alpha administered on DO and 1 previously-used CIDR (CIDR3) inserted for 5 days (Small-P-4-dose-D5; n = 8) or 6 days (Small-P-4-dose-D6; n = 8), CIDR1 inserted on DO and removed plus PGF(2 alpha) on D5 (Large-P-4-dose-pmestrus (PE); n = 7), and CIDR3 and PGF(2 alpha) on DO and 1, CIDR3 removed plus PGF(2 alpha) on D5 (Small-P-4-dose-PE; n = 7). Duration of P-4 treatment (D5 compared to D6) affected abundances of CYP19A1 mRNA transcripts, with there being greater abundances on D6 than D5 (P <= 0.05). Heifers treated with the large dose of P-4 had a smaller dominant follicle, less serum and intra-follicular estradiol (E-2) concentrations (P <= 0.05) and lesser LHCGR, CYP19A1, and HSD3B1 transcript abundances (P <= 0.05). Heifers treated to induce PE had a larger follicle diameter (P = 0.09), greater intra-follicular E-2 concentrations and larger abundances of CYP19A1 mRNA transcript (P <= 0.05) than heifers of the D6 group. Overall, treatment with larger doses of P-4 resulted in lesser abundances of LHCGR, HSD3B1, and CYP19A1 mRNA transcripts; thus, potentially leading to development of smaller dominant follicles and lesser E-2 concentrations. (AU)

FAPESP's process: 13/17181-8 - Effect of progesterone on the expression of genes related to follicular development in granulosa cells of Nellore heifers
Grantee:Henrique Pedro Dias
Support Opportunities: Scholarships in Brazil - Master