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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Cellulose nanofibers production using a set of recombinant enzymes

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Author(s):
Rossi, Bruno R. [1] ; Pellegrini, Vanessa O. A. [1] ; Cortez, Anelyse A. [1] ; Chiromito, Emanoele M. S. [2] ; Carvalho, Antonio J. F. [2] ; Pinto, Lidiane O. [3] ; Rezende, Camila A. [3] ; Mastelaro, Valmor R. [1] ; Polikarpov, Igor [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sao Carlos Inst Phys, Av Trabalhador Saocarlense 400, BR-13566590 Sao Carlos, SP - Brazil
[2] Univ Sao Paulo, Mat Sci & Engn Dept, Sao Carlos Sch Engn, Av Joao Dagnone 1100, BR-13563120 Sao Carlos, SP - Brazil
[3] Univ Estadual Campinas, Inst Chem, POB 6154, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Carbohydrate Polymers; v. 256, MAR 15 2021.
Web of Science Citations: 0
Abstract

Cellulose nanofibers (CNF) are renewable and biodegradable nanomaterials with attractive barrier, mechanical and surface properties. In this work, three different recombinant enzymes: an endoglucanase, a xylanase and a lytic polysaccharide monooxygenase, were combined to enhance cellulose fibrillation and to produce CNF from sugarcane bagasse (SCB). Prior to the enzymatic catalysis, SCB was chemically pretreated by sodium chlorite and KOH, while defibrillation was accomplished via sonication. We obtained much longer (mu m scale length) and more thermostable (resisting up to 260 degrees C) CNFs as compared to the CNFs prepared by TEMPO-mediated oxidation. Our results showed that a cooperative action of the set of hydrolytic and oxidative enzymes can be used as a ``green{''} treatment prior to the sonication step to produce nanofibrillated cellulose with advanced properties. (AU)

FAPESP's process: 15/13684-0 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation
Grantee:Igor Polikarpov
Support Opportunities: Research Projects - Thematic Grants