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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Ischemia-induced retinal injury is attenuated by Neurovespina, a peptide from the venom of the social wasp Polybia occidentalis

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Author(s):
Dourado, Lays Fernanda Nunes [1] ; Silva, Carolina Nunes [1] ; Anjos, Lilian Carneiro dos [2] ; Mortari, Marcia Renata [2] ; Silva-Cunha, Armando [1] ; Fialho, Silvia Ligorio [3]
Total Authors: 6
Affiliation:
[1] Univ Fed Minas Gerais, Fac Farm, Campus Pampulha, Av Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG - Brazil
[2] Univ Brasilia, Inst Ciencias Biol, Dept Ciencias Fisiol, Lab Neurofarmacol, Brasilia, DF - Brazil
[3] Fundacao Ezequiel Dias, Diretoria Pesquisa & Desenvolvimento, Rua Conde Pereira Carneiro 80, BR-30510010 Belo Horizonte, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Neuropeptides; v. 85, FEB 2021.
Web of Science Citations: 0
Abstract

Neurovespina is a synthetic peptide modified from Occidentalin-1202, a nine amino acid residue peptide isolated from the venom of the social wasp Polybia occidentalis. Previous studies showed that this peptide has a neuroprotective effect on the central nervous system, but its action on the eye has not been explored. So, the objective of this work was to investigate the neuroprotective effect of Neurovespina on the retina and its angiogenic potential in the chicken chorioallantoic membrane (CAM). Retinal ischemia was induced in rats by acute elevation of intraocular pressure (IOP). Electroretinography (ERG) measurements, histopathological and immunohistochemical analysis, and transmission electronic microscopy (TEM) records were performed to check the neuroprotection effect of Neurovespina in the retina of the animals. The angiogenic activity of the peptide was investigated by CAM assay. The results showed that Neurovespina was able to reduce the effects induced by ischemic injury, preventing the reduction of a- and b-waves in the scotopic ERG. Histopathological and immunohistochemistry assays showed that Neurovespina, mainly at 60 mu g/ml, protected all layers of the retina. The CAM assay revealed that the peptide promoted the reduction of CAM vessels. So, Neurovespina was able to protect retinal cells from ischemic insult and has an antiangiogenic effect, which can be considered as a promising neuroprotective agent for intravitreal application. (AU)

FAPESP's process: 14/50928-2 - INCT 2014: Pharmaceutical Nanotechnology: a transdisciplinary approach
Grantee:Maria Vitória Lopes Badra Bentley
Support Opportunities: Research Projects - Thematic Grants