Resistance Training Modulates the Matrix Metalloproteinase-2 Activity in Different Trabecular Bones in Aged Rats

Author(s)

de Sousa Neto, Ivo Vieira ^[1] ; Quaglioti Durigan, Joao Luiz ^{[2, 1]} ; de Farias Junior, Goncalo Carreiro ^[2] ; Bogni, Fabio Henrique ^[3] ; Ruivo, Amanda Lima ^[1] ; de Araujo, Juliana Oliveira ^[1] ; Nonaka, Keico Okino ^[3] ; Selistre-de-Araujo, Heloisa ^[3] ; Marqueti, Rita de Cassia ^{[2, 1]}

Affiliation

^[1] Univ Brasilia, Lab Mol Anal, Grad Program Sci & Technol Hlth, Brasilia, DF - Brazil

^[2] Univ Brasilia, Grad Program Rehabil Sci, Brasilia, DF - Brazil

^[3] Univ Fed Sao Carlos, Dept Physiol Sci, Sao Paulo - Brazil

• Document type: Journal article
• Source: CLINICAL INTERVENTIONS IN AGING; v. 16, p. 71-81, 2021.
• Web of Science Citations: 0

Abstract

Background: Aging decreases osteogenic ability, inducing harmful effects on the bone extracellular matrix (ECM), while exercise training has been indicated as a tool to counteract bone disorders related to advancing age. The modulation of bone ECM is regulated by several types of matrix metalloproteinase (MMP), however, MMP-2 activity in different trabecular bones in response to resistance training (RT) has been neglected. Remodeling differs in different bones under the application of the same mechanical loading. Thus, we investigated the effects of 12 weeks of RT on MMP-2 activity in the lumbar vertebra (L6), tibia, and femur of young (3 months) and older rats (21 months). Methods: Twenty Wistar rats were divided into four groups (five animals per group): young sedentary or trained and older sedentary or trained. The 12-week RT consisted of climbing a 1.1-m vertical ladder three times per week with progressive weights secured to the animals' tails. The animals were killed 48 h after the end of the experimental period. The MMP-2 activity was assessed by the zymography method. Results: The aging process induced lower MMP-2 activity in the lumbar vertebrae and tibia (p=0.01). RT upregulated pro, intermediate, and active MMP-2 activity in the tibia of young rats (p=0.001). RT also upregulated pro and active MMP-2 activity in the lumbar vertebrae and tibia with advancing age (p=0.01). There was no significant difference (p>0.05) between groups for MMP-2 of the femur, regardless of age and RT. Conclusion: The aging process impairs MMP-2 activity, but RT is a potential therapeutic approach to minimize the deleterious effects of ECM degeneration in different aged bones. Distinct MMP-2 responses to exercise training may result in specific remodeling processes. (AU)

• FAPESP's process: 11/11229-3 - Aging-related alterations in extracellular matrix and its adaptations to strength training in calcaneal tendon of rats: molecular, cellular, and biomechanical approach
• Grantee: Heloisa Sobreiro Selistre de Araújo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/00798-2 - The extracellular matrix in aging, exercise and in the tumor microenvironment
• Grantee: Heloisa Sobreiro Selistre de Araújo
• Support Opportunities: Research Projects - Thematic Grants