Essential Oils as Natural Fungicides to Control Rhizopus stolonifer-Induced Spoiled of Strawberries

Author(s)

Oliveira Filho, Josemar Goncalves ^[1] ; Silva, Guilherme da Cruz ^[2] ; Egea, Mariana Buranelo ^[3] ; Cordeiro de Azeredo, Henriette Monteiro ^{[4, 5]} ; Ferreira, Marcos David ^[5]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Araraquara, SP - Brazil

^[2] Univ Fed Sao Carlos, Dept Biotechnol, Sao Carlos - Brazil

^[3] Goiano Fed Inst Educ Sci & Technol, Campus Rio Verde, Rio Verde, Go - Brazil

^[4] Embrapa Agroindastria Trop, Brazilian Agr Res Corp, Fortaleza, Ceara - Brazil

^[5] Embrapa Instrumentacao, Brazilian Agr Res Corp, Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY; v. 11, n. 5, p. 13244-13251, OCT 15 2021.
• Web of Science Citations: 0

Abstract

Strawberry is a highly desirable fruit with a unique taste and a good source of bioactive compounds beneficial to human health. However, it has a short post-harvest shelf life, mainly due to the soft rot caused by Rhizopus stolonifer. This study aimed to evaluate the antimicrobial properties of essential oils (EOs) of Mentha piperita, Cymbopogon martinii, Cinnamomum camphora, and Mentha spicata using spore germination and micro-well dilution assays, and to test the effects of the vapor-phase application of M. spicata and C. martini on the incidence and severity of soft rot in strawberry artificially inoculated with R. stolonifer. In in vitro tests, C. martinii and M. spicata EOs were the most effective, inhibiting more than 95% of the spore germination. Additionally, in the microwell dilution test, these EOs had the lowest minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) (5 and 10 mu g/mL, respectively), and for the microwell dilution test, the lowest MIC (5 mu g/mL and 10 mu g/mL, respectively) and MFC (10 mu g/mL for both). High in vivo inhibitory effects of M. spicata and C. martinii EOs were observed at 10% concentration, with 100 and 78% reduction, respectively, in the R. stolonifer-induced spoilage. Our results suggest that C. martinii and M. spicata EOs can be used as efficient natural fungicides and can be an alternative to synthetic fungicides for preserving fresh strawberries from soft rot. (AU)

• FAPESP's process: 18/24612-9 - Application of nanoemulsions with essential oils as natural coatings for application in post-harvesting strawberry conservation
• Grantee: Josemar Gonçalves de Oliveira Filho
• Support Opportunities: Scholarships in Brazil - Doctorate