Combining Celery Oleoresin, Limonene and Rhamnolipid as New Strategy to Control Endospore-Forming Bacillus cereus

Author(s)

Bertuso, Paula de Camargo ^[1] ; Drappe Mayer, Debora M. ^[1] ; Nitschke, Marcia ^[2]

Affiliation

^[1] Univ Sao Paulo, Interunits Grad Program Bioengn EESC FMRP IQSC, Trabalhador Sao Carlense Av 400, BR-13566590 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Chem IQSC, Trabalhador Sao Carlense Av 400, POB 780, BR-13560970 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: FOODS; v. 10, n. 2 FEB 2021.
• Web of Science Citations: 0

Abstract

Foodborne diseases (FBD) are a great problem worldwide, leading millions of people to seek medical help and to significant economic losses for industry. Among the agents implicated in FDB is Bacillus cereus, a Gram-positive, toxigenic and endospore-forming bacterium. In this study, rhamnolipid (RL) biosurfactant, celery oleoresin (OR) and limonene (LN) were evaluated as bio-based alternatives for controlling the growth of vegetative cells and endospores of B. cereus. To address their antimicrobial activity, the compounds were tested separately and in combination. Results demonstrate that, when combined with RL, both OR and LN have lower minimal inhibitory concentration (MIC) values and increased endospore inhibition potential. A percentage of endospore inhibition from 73% to 98%, corresponding to a 2.8-3.6 log reduction in spore outgrowth, was observed. RL inhibited B. cereus growth and endospore germination and potentially enhanced the antimicrobial efficacy of the natural hydrophobic compounds tested. (AU)

• FAPESP's process: 19/14405-9 - Exploring the potential of Rhamnolipid on controlling food pathogens: effect of ionic strength and biopolymers
• Grantee: Marcia Nitschke
• Support Opportunities: Regular Research Grants