Fe3O4 nanoparticles and Rhizobium inoculation enhance nodulation, nitrogen fixation and growth of common bean plants grown in soil

Author(s)

De Souza-Torres, Angel ^[1] ; Govea-Alcaide, Ernesto ^[2] ; Gomez-Padilla, Ernesto ^[3] ; Masunaga, Sueli H. ^[4] ; Effenberger, Fernando B. ^[5] ; Rossi, Liane M. ^[6] ; Lopez-Sanchez, Raul ^[3] ; Jardim, Renato F. ^[7]

Affiliation

^[1] Granma Univ, Dept Phys & Math, Apartado 21, Bayamo 85149, Granma - Cuba

^[2] Univ Fed Amazonas, Dept Phys, Manaus, Amazonas - Brazil

^[3] Granma Univ, Agr Fac, Ctr Plant Biotechnol Studies, Bayamo - Cuba

^[4] Ctr Univ FEI, Dept Phys, Sb Campo, SP - Brazil

^[5] Ctr Univ FEI, Dept Chem, Sb Campo, SP - Brazil

^[6] Univ Sao Paulo, Inst Chem, Sao Paulo - Brazil

^[7] Univ Sao Paulo, Inst Phys, Sao Paulo - Brazil

• Document type: Journal article
• Source: RHIZOSPHERE; v. 17, MAR 2021.
• Web of Science Citations: 0

Abstract

The effects of Fe3O4 nanoparticles (NPs) and Rhizobium inoculation on nodulation, nitrogen fixation and plant growth of common bean (cv. Red Guama, Phaseolus vulgaris) plants were investigated in growth chambers. Plants were exposed to: Fe3O4 NPs (2000 mg/L) (T1), Rhizobium inoculation (T2) and Fe(3)O(4 )NPs + Rhizobium inoculation (T3); non-treated plants were considered as controls. Harvested 35-day-old treated plants showed improved symbiotic performance including increased nitrogenase activity (51.2-90.7%), nodule leghaemoglobin (44.8-80.9%) and iron content (83.4-84.2%), number of active nodules per plant (58.7-122%) and nodule dry weight (40.2-70.6%). This resulted in enhanced symbiotic nitrogen fixation,and increased shoot (26.5-50.2%) and mot (24.1-48.2%) total nitrogen content in treated plants in comparison with the controls. The best result was obtained using treatment T3. Furthermore, Fe(3)O(4 )NPs were taken up by bean plants in treatments T1 and T3, and these accumulated in their organs, including in nodules. All treatments led to an increase in mot (51.9-79.8%) and shoot (27.5-52.7%) lengths, in leaf area (10.9-16.8%) and in root (10.1-17.8%), stem (9.8-12.7%) and leaf dry weight (8-17.3%) compared to control plants. Thus applied treatments have the potential to improve common bean plant growth through enhancement of nodulation and nitrogen fixation during vegetative growth.This study also provides strong evidence that the presence Fe3O4 NPs in nodules improves the symbiotic performance between Rhizobium (leguminosarum CF1 strain) and the common bean plant, due to enhanced nodulation and nitrogen fixation. (AU)

• FAPESP's process: 14/19245-6 - Search for novel superconducting materials
• Grantee: Renato de Figueiredo Jardim
• Support Opportunities: Scholarships abroad - Research
• FAPESP's process: 14/12392-3 - Magnetometric tracking of magnetic nanoparticles in common bean plants grown in soil
• Grantee: Renato de Figueiredo Jardim
• Support Opportunities: Research Grants - Visiting Researcher Grant - International