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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Disturbed Blood Flow Acutely Increases Endothelial Microparticles and Decreases Flow Mediated Dilation in Patients With Heart Failure With Reduced Ejection Fraction

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Author(s):
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Silva, Thiago O. C. [1] ; Sales, Allan R. K. [1, 2] ; Araujo, Gustavo S. M. [1] ; Fonseca, Guilherme W. P. [1] ; Braga, Pedro G. S. [1] ; Faria, Diego [2] ; Rocha, Helena N. M. [3] ; Rocha, Natalia G. [3] ; Lima, Marta F. [1] ; Mady, Charles [1] ; Negrao, Carlos E. [4, 1] ; Alves, Maria Janieire N. N. [1]
Total Authors: 12
Affiliation:
[1] Univ Sao Paulo, Sch Med, Inst Heart, Sao Paulo - Brazil
[2] Dor Inst Res & Educ, Sao Paulo - Brazil
[3] Fluminense Fed Univ, Dept Physiol & Pharmacol, Niteroi, RJ - Brazil
[4] Univ Sao Paulo, Sch Phys Educ & Sport, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: FRONTIERS IN PHYSIOLOGY; v. 12, MAR 11 2021.
Web of Science Citations: 0
Abstract

Introduction Disturbed blood flow, characterized by high retrograde and oscillatory shear rate (SR), is associated with a proatherogenic phenotype. The impact of disturbed blood flow in patients with heart failure with reduced ejection fraction (HFrEF) remains unknown. We tested the hypothesis that acute elevation to retrograde and oscillatory SR provoked by local circulatory occlusion would increase endothelial microparticles (EMPs) and decrease brachial artery flow-mediated dilation (FMD) in patients with HFrEF. Methods Eighteen patients with HFrEF aged 55 +/- 2 years, with left ventricular ejection fraction (LVEF) 26 +/- 1%, and 14 control subjects aged 49 +/- 2 years with LVEF 65 +/- 1 randomly underwent experimental and control sessions. Brachial artery FMD (Doppler) was evaluated before and after 30 min of disturbed forearm blood flow provoked by pneumatic cuff (Hokanson) inflation to 75 mm Hg. Venous blood samples were collected at rest, after 15 and 30 min of disturbed blood flow to assess circulating EMP levels (CD42b-/CD31+; flow cytometry). Results At rest, FMD was lower in patients with HFrEF compared with control subjects (P < 0.001), but blood flow patterns and EMPs had no differences (P > 0.05). The cuff inflation provoked a greater retrograde SR both groups (P < 0.0001). EMPs responses to disturbed blood flow significantly increased in patients with HFrEF (P = 0.03). No changes in EMPs were found in control subjects (P > 0.05). Disturbed blood flow decreased FMD both groups. No changes occurred in control condition. Conclusion Collectively, our findings suggest that disturbed blood flow acutely decreases FMD and increases EMP levels in patients with HFrEF, which may indicate that this set of patients are vulnerable to blood flow disturbances. (AU)

FAPESP's process: 15/22814-5 - Cancer and heart: new paradigms of diagnosis and treatment
Grantee:Carlos Eduardo Negrão
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/11671-6 - Effect of high intensity interval training versus continuous moderate in the peripheral reflex mechanisms of control of the sympathetic activity in patients with heart failure
Grantee:Allan Robson Kluser Sales
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/25613-6 - Habitual aerobic exercise decreases endothelin-1 and restores insulin-induced vasodilation in patients with type 2 diabetes: role shear stress
Grantee:Allan Robson Kluser Sales
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor