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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Breast Cancer Promotes Cardiac Dysfunction Through Deregulation of Cardiomyocyte Ca2+-Handling Protein Expression That is Not Reversed by Exercise Training

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Author(s):
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da Costa, Tassia S. R. [1, 2] ; Urias, Ursula [3, 1] ; Negrao, V, Marcelo ; Jordao, Camila P. [1] ; Passos, Clevia S. [1] ; Gomes-Santos, Igor L. [4, 1, 5] ; Salemi, Vera Maria C. [1] ; Camargo, Anamaria A. [6] ; Brum, Patricia C. [3] ; Oliveira, Edilamar M. [3] ; Hajjar, Ludhmila A. [1, 2] ; Chammas, Roger [2] ; Filho, Roberto K. [1] ; Negrao, Carlos E. [3, 1]
Total Authors: 14
Affiliation:
[1] Univ Sao Paulo, Heart Inst InCor Hosp Clin, Fac Med, Sao Paulo - Brazil
[2] Univ Sao Paulo, Canc Inst State Sao Paulo, Fac Med, Hosp Clin, Sao Paulo - Brazil
[3] Univ Sao Paulo, Sch Phys Educ & Sport, Sao Paulo - Brazil
[4] Harvard Med Sch, Boston, MA 02115 - USA
[5] Massachusetts Gen Hosp, Edwin L Steele Labs Tumor Biol, Dept Radiat Oncol, Boston, MA 02114 - USA
[6] Hosp Sirio Libanes, Ctr Oncol Mol, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: JOURNAL OF THE AMERICAN HEART ASSOCIATION; v. 10, n. 5 MAR 2 2021.
Web of Science Citations: 1
Abstract

Background Patients treated for breast cancer have a high incidence of cardiovascular complications. In this study, we evaluated the impact of breast cancer on cardiac function and cardiomyocyte Ca2+-handling protein expression. We also investigated whether exercise training (ET) would prevent these potential alterations. Methods and Results Transgenic mice with spontaneous breast cancer (mouse mammary tumor virus-polyomavirus middle T antigen {[}MMTV-PyMT+], n=15) and littermate mice with no cancer (MMTV-PyMT-, n=14) were studied. For the ET analysis, MMTV-PyMT+ were divided into sedentary (n=10) and exercise-trained (n=12) groups. Cardiac function was evaluated by echocardiography with speckle-tracking imaging. Exercise tolerance test was conducted on a treadmill. Both studies were performed when the tumor became palpable and when it reached 1 cm(3). After euthanasia, Ca2+-handling protein expression (Western blot) was evaluated. Exercise capacity was reduced in MMTV-PyMT+ compared with MMTV-PyMT- (P-interaction=0.031). Longitudinal strain (P-group <0.001) and strain rate (P-group=0.030) were impaired. Cardiomyocyte phospholamban was increased (P=0.011), whereas phospho-phospholamban and sodium/calcium exchanger were decreased (P=0.038 and P=0.017, respectively) in MMTV-PyMT+. No significant difference in sarcoplasmic or endoplasmic reticulum calcium 2 ATPase (SERCA2a) was found. SERCA2a/phospholamban ratio was reduced (P=0.007). ET was not associated with increased exercise capacity. ET decreased left ventricular end-systolic diameter (P-group=0.038) and end-diastolic volume (P-group=0.026). Other morphological and functional cardiac parameters were not improved by ET in MMTV-PyMT+. ET did not improve cardiomyocyte Ca2+-handling protein expression. Conclusions Breast cancer is associated with decreased exercise capacity and subclinical left ventricular dysfunction in MMTV-PyMT+, which is at least partly associated with dysregulation of cardiomyocyte Ca2+ handling. ET did not prevent or reverse these changes. (AU)

FAPESP's process: 15/22814-5 - Cancer and heart: new paradigms of diagnosis and treatment
Grantee:Carlos Eduardo Negrão
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 15/19076-2 - Effect of physical exercise on the survival of circulating tumor cells and tumor formation
Grantee:Clévia dos Santos Passos
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/13690-8 - Role of exercise training on prevention of cardiotoxicity provoked by doxorubicin
Grantee:Igor Lucas Gomes dos Santos
Support Opportunities: Scholarships in Brazil - Post-Doctoral