Hypothalamic Microglial Heterogeneity and Signature under High Fat Diet-Induced Inflammation

Author(s)

Mendes, Natalia Ferreira ^{[1, 2]} ; Jara, Carlos Poblete ^{[1, 2]} ; Zanesco, Ariane Maria ^{[2, 3]} ; de Araujo, Eliana Pereira ^{[1, 2]}

Affiliation

^[1] Univ Estadual Campinas, Fac Nursing, BR-13083887 Campinas - Brazil

^[2] Univ Estadual Campinas, Lab Cell Signaling, Obes & Comorbid Res Ctr, BR-13083864 Campinas - Brazil

^[3] Univ Estadual Campinas, Sch Med Sci, BR-13083887 Campinas - Brazil

• Document type: Review article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 22, n. 5 MAR 2021.
• Web of Science Citations: 3

Abstract

Under high-fat feeding, the hypothalamus atypically undergoes pro-inflammatory signaling activation. Recent data from transcriptomic analysis of microglia from rodents and humans has allowed the identification of several microglial subpopulations throughout the brain. Numerous studies have clarified the roles of these cells in hypothalamic inflammation, but how each microglial subset plays its functions upon inflammatory stimuli remains unexplored. Fortunately, these data unveiling microglial heterogeneity have triggered the development of novel experimental models for studying the roles and characteristics of each microglial subtype. In this review, we explore microglial heterogeneity in the hypothalamus and their crosstalk with astrocytes under high fat diet-induced inflammation. We present novel currently available ex vivo and in vivo experimental models that can be useful when designing a new research project in this field of study. Last, we examine the transcriptomic data already published to identify how the hypothalamic microglial signature changes upon short-term and prolonged high-fat feeding. (AU)

• FAPESP's process: 19/00330-7 - Effect of palmitate treatment in pc1/3 expression in a hypothalamic cell lineage
• Grantee: Ariane Maria Zanesco
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 17/22511-8 - Role of microglial cells in the high-fat diet-induced hypothalamic inflammation
• Grantee: Natália Ferreira Mendes
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 16/17810-3 - Evaluation of TGF-b1 function in the hypothalamus of animals with obesity and diabetes induced by high-fat diet
• Grantee: Natália Ferreira Mendes
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 13/07607-8 - OCRC - Obesity and Comorbidities Research Center
• Grantee: Licio Augusto Velloso
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC