| Full text | |
| Author(s): |
Castilho, Maiara L.
[1, 2]
;
Jesus, Viviane P. S.
[1]
;
Vieira, Paula F. A.
[1]
;
Hewitt, Kevin C.
[3]
;
Raniero, L.
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, Lab Nanossensores, UNIVAP, Av Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[2] Univ Vale Paraiba, Inst Pesquisa & Desenvolvimento, Lab Bionanotecnol, UNIVAP, Av Shishima Hifumi 2911, BR-12244000 Sao Jose Dos Campos, SP - Brazil
[3] Dalhousie Univ, Dept Phys & Atmospher Sci, 6310 Coburg Rd, Halifax, NS B3H 4R2 - Canada
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Photodiagnosis and Photodynamic Therapy; v. 33, MAR 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
To develop a treatment modality for triple-negative breast cancer, we investigated the efficacy of a bifunctional theranostic nanoprobes (BN) during Photodynamic Therapy (PDT) on human breast carcinoma and normal human cells. The BN is a 21 nm gold nanoparticles functionalized with Chlorin e6 (Ce6) and Epidermal Growth Factor (EGF). Attachment to gold nanoparticle stabilizes Ce6 while EGF acts as a cancer cell targeting agent. Fluorescence Spectroscopy and Confocal Fluorescence Microscopy revealed a gradual uptake of nanoprobes into cancer cells at an average rate of 63 BN/min. Cell viability assays showed that 0.2 ?g/mL BN concentration was highly cytotoxic to cancer cells (86 %), but not normal cells. At this concentration, 58 % cancer cells were necrotic and 38 % apoptotic, while the reactive oxygen species (ROS) was 9-fold higher in cancer cells compared to normal. Overall, results suggest that BN mediated PDT can achieve targeted cancer cell death with high efficiency. (AU) | |
| FAPESP's process: | 17/07519-2 - TREATMENT OF BREAST CANCER IN ANIMAL MODEL VIA PHOTODYNAMIC THERAPY ASSOCIATED WITH BIFUNCTIONAL NANOprobes |
| Grantee: | Leandro José Raniero |
| Support Opportunities: | Regular Research Grants |