Guapiacu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil

Author(s):Show less -

Ribeiro, Geovani de Oliveira ^[1] ; da Costa, Antonio Charlys ^[2] ; Gill, Danielle Elise ^[2] ; D'Athaide Ribeiro, Edcelha Soares ^[3] ; Rego, Marlisson Octavio da S. ^[3] ; Monteiro, Fred Julio Costa ^[3] ; Villanova, Fabiola ^[1] ; Nogueira, Juliana Silva ^[4] ; Maeda, Adriana Yurika ^[4] ; de Souza, Renato Pereira ^[4] ; Tahmasebi, Roozbeh ^[2] ; Morais, Vanessa S. ^[2] ; Pandey, Ramendra Pati ^[5] ; Raj, V. Samuel ^[5] ; Salloum Scandar, Sirle Abdo ^[6] ; da Silva Vasami, Fernanda Gisele ^[4] ; D'Agostino, Leandro Guaraglia ^[4] ; Maiorka, Paulo Cesar ^[7] ; Deng, Xutao ^{[8, 9]} ; Nogueira, Mauricio Lacerda ^[10] ; Sabino, Ester Cerdeira ^[2] ; Delwart, Eric ^{[8, 9]} ; Leal, Elcio ^[1] ; Cunha, Mariana Sequetin ^{[2, 4]}

Affiliation

^[1] Fed Univ Para, Inst Biol Sci, BR-66075000 Belem, Para - Brazil

^[2] Univ Sao Paulo, Inst Trop Med, BR-05403000 Sao Paulo, SP - Brazil

^[3] Hlth Surveillance Superintendence Amapa, Publ Hlth Lab Amapa LACEN AP, Rua Tancredo Neves 1-118, BR-68905230 Macapa, AP - Brazil

^[4] Adolfo Lutz Inst, Virol Ctr, Vector Borne Dis Lab, BR-01246000 Sao Paulo, SP - Brazil

^[5] SRM Univ, Delhi NCR, Ctr Drug Design Discovery & Dev C4D, Sonepat, Haryana - India

^[6] Superintendence Control Endem Dis SUCEN, Sao Jose Do Rio Preto, SP - Brazil

^[7] Univ Sao Paulo, Fac Vet Med, Dept Pathol, Sao Paulo, SP - Brazil

^[8] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 - USA

^[9] Vitalant Res Inst, 270 Masonic Ave, San Francisco, CA 94118 - USA

^[10] Fac Med Sao Jose do Rio Preto FAMERP, Sao Jose Do Rio Preto, SP - Brazil

• Document type: Journal article
• Source: SCIENTIFIC REPORTS; v. 11, n. 1 FEB 25 2021.
• Web of Science Citations: 2

Abstract

Classical insect-flaviviruses (cISFVs) and dual host-related insect-specific flavivirus (dISFV) are within the major group of insect-specific flavivirus. Remarkably dISFV are evolutionarily related to some of the pathogenic flavivirus, such as Zika and dengue viruses. The Evolutionary relatedness of dISFV to flavivirus allowed us to investigate the evolutionary principle of host adaptation. Additionally, dISFV can be used for the development of flavivirus vaccines and to explore underlying principles of mammalian pathogenicity. Here we describe the genetic characterization of a novel putative dISFV, termed Guapiacu virus (GUAPV). Distinct strains of GUAPV were isolated from pools of Aedes terrens and Aedes scapularis mosquitoes. Additionally, we also detected viral GUAPV RNA in a plasma sample of an individual febrile from the Amazon region (North of Brazil). Although GUAPV did not replicate in tested mammalian cells, 3 ` UTR secondary structures duplication and codon usage index were similar to pathogenic flavivirus. (AU)

• FAPESP's process: 19/21706-5 - Viral metagenomics of mosquitoes captured in the State of São Paulo
• Grantee: Vanessa dos Santos Morais
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 13/21719-3 - Epidemiological study of dengue (serotypes1-4) in a cohort of São José do Rio Preto, São Paulo, Brazil, during 2014-2018
• Grantee: Maurício Lacerda Nogueira
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/01735-2 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Ester Cerdeira Sabino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/23645-4 - Study of pathological alterations in situations of expert witness examination of animals
• Grantee: Paulo César Maiorka
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/00021-9 - Viral metagenomics to track, explain and predict the transmission and spatiotemporal spread of Dengue and Chikungunya viruses in Brazil
• Grantee: Antonio Charlys da Costa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral