Joa yellow blotch-associated virus, a new alphanucleorhabdovirus from a wild solanaceous plant in Brazil

Author(s)

Dietzgen, Ralf G. ^{[1, 2]} ; Bejerman, Nicolas E. ^{[3, 4]} ; Mei, Yongyu ^{[1, 2]} ; Jee, Charmaine Lim Jing ^{[1, 2]} ; Chabi-Jesus, Camila ^[5] ; Freitas-Astua, Juliana ^{[5, 6]} ; Veras, Solange M. ^[7] ; Kitajima, Elliot W. ^[8]

Affiliation

^[1] Univ Queensland, Sch Chem & Mol Biosci, St Lucia, Qld 4072 - Australia

^[2] Univ Queensland, Queensland Alliance Agr & Food Innovat, St Lucia, Qld 4072 - Australia

^[3] Inst Nacl Tecnol Agr IPAVE CIAP INTA, Ctr Invest Agr, Inst Patol Vegetal, RA-5020 Cordoba - Argentina

^[4] Consejo Nacl Invest Cient & Tecn, Unidad Fitopatol & Modelizac Agr, Buenos Aires, DF - Argentina

^[5] Inst Biol, BR-04014900 Sao Paulo - Brazil

^[6] Embrapa Cassava & Fruits, BR-44380000 Cruz Das Almas, BA - Brazil

^[7] Univ Fed Amazonas, Dept Ciencias Fundamentais & Desenvolvimento Agr, Av Gen Rodrigo Octavio Jordao Ramos 1200, BR-69067005 Manaus, Amazonas - Brazil

^[8] ESALQ USP, Dept Fitopatol & Nematol, BR-13418900 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: ARCHIVES OF VIROLOGY; v. 166, n. 6 MAR 2021.
• Web of Science Citations: 0

Abstract

We identified a novel plant rhabdovirus infecting native joa (Solanum aculeatissimum) plants in Brazil. Infected plants showed yellow blotches on the leaves, and typical enveloped bacilliform rhabdovirus particles associated with the nucleus were seen in thin sections by electron microscopy. The virus could be graft-transmitted to healthy joa and tomato plants but was not mechanically transmissible. RT-PCR using degenerate plant rhabdovirus L gene primers yielded an amplicon from extracted total RNA, the sequence of which was similar to those of alphanucleorhabdoviruses. Based on close sequence matches, especially with the type member potato yellow dwarf virus (PYDV), we adopted a degenerate-primer-walking strategy towards both genome ends. The complete genome of joa yellow blotch-associated virus (JYBaV) is comprised of 12,965 nucleotides, is less than 75% identical to that of its closest relative PYDV, and clusters with PYDV and other alphanucleorhabdoviruses in L protein phylogenetic trees, suggesting that it should be taxonomically classified in a new species in the genus Alphanucleorhabdovirus, family Rhabdoviridae. The genome organization of JYBaV is typical of the `PYDV-like' subgroup of alphanucleorhabdoviruses, with seven genes (N-X-P-Y-M-G-L) separated by conserved intergenic regions and flanked by partly complementary 3' leader and 5' trailer regions. (AU)

• FAPESP's process: 17/50222-0 - Understanding the molecular biology and ecology of plant-virus-vector relationships: towards sustainable, integrated virus management strategies
• Grantee: Juliana de Freitas Astúa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/18910-4 - Diversity of molecular pathogens (viruses and viroids) present in Brazil. Phase II. 2018
• Grantee: Elliot Watanabe Kitajima
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants