Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bacteriophages that infect Gram-negative bacteria as source of signal-arrest-release motif lysins

Full text
Pardini Gontijo, Marco Tulio [1] ; Pereira Vidigal, Pedro Marcus [2] ; Soto Lopez, Maryoris Elisa [3] ; Brocchi, Marcelo [1]
Total Authors: 4
[1] Univ Estadual Campinas UNICAMP, Inst Biol, Dept Genet Evoluc Microbiol & Imunol, Rua Monteiro Lobato 255, BR-13083862 Sao Paulo - Brazil
[2] Univ Fed Vicosa UFV, Brazil Elect Address Pmvidigal Gmailcom, Vila Gianetti, Casa 21, Campus UFV, BR-36570900 Vicosa, MG - Brazil
[3] Univ Cordoba UNICORDOBA, Dept Ingn Alimentos, Carrera 6 77-305, Monteria 230002 - Colombia
Total Affiliations: 3
Document type: Journal article
Source: Research in Microbiology; v. 172, n. 2 MAR 2021.
Web of Science Citations: 0

Treatment of infections caused by multidrug-resistant (MDR) Gram-negative bacteria is challenging, a potential solution for which is the use of bacteriophage-derived lytic enzymes. However, the exogenous action of bacteriophage lysins against Gram-negative bacteria is hindered due to the presence of an impermeable outer membrane in these bacteria. Nevertheless, recent research has demonstrated that some lysins are capable of permeating the outer membrane of Gram-negative bacteria with the help of signal peptides. In the present study, we investigated the genomes of 309 bacteriophages that infect Gram-negative pathogens of clinical interest in order to determine the evolutionary markers of signal peptide-containing lysins. Complete genomes displayed 265 putative lysins, of which 17 (6.41%) con-tained signal-arrest-release motifs and 41 (15.47%) contained cleavable signal peptides. There was no apparent relationship between host specificity and lysin diversity. Nevertheless, the evolution of lysin genes might not be independent of the rest of the bacteriophage genome once pan-genome clustering and lysin diversity appear to be correlated. In addition, signal peptide-and signal-arrest-release-containing lysins were monophyletically distributed in the protein cladogram, suggesting that the nat-ural selection of holin-independent lysins is divergent. Our study screened 58 (21.89%) out of 265 po-tential candidates for in vitro experimentation against MDR bacteria. (c) 2020 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU)

FAPESP's process: 17/10051-2 - Biotechnological potential and phenotypic and molecular characterization of Salmonella enterica mutants
Grantee:Marcelo Brocchi
Support type: Regular Research Grants
FAPESP's process: 20/01535-9 - Comparative genomics and antimicrobial activity of recombinant phage endolisins against multi-resistant gram-negative bacteria
Grantee:Marco Túlio Pardini Gontijo
Support type: Scholarships in Brazil - Master