Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Photobiomodulation effects on photodynamic therapy in HNSCC cell lines

Full text
Author(s):
de Faria, Clara M. G. [1] ; Costa, Camilla S. [1] ; Bagnato, Vanderlei S. [1]
Total Authors: 3
Affiliation:
[1] Univ Sao Paulo, Sao Carlos Inst Phys, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 217, APR 2021.
Web of Science Citations: 0
Abstract

A combination of metabolic modifications by light stimulus and photodynamic action is very attractive. Photobiomodulation therapy (PBMT) comprehends a vast range of applications and has been shown to be suitable to ease morbidities caused by chemotherapy and radiation, such as mucositis and dermatitis. The current study investigates the effects of near-infrared PBMT combined with porphyrin-based photodynamic therapy (PDT) in squamous cell carcinoma cell lines SCC-25 and SCC-4. The aim is to evaluate the potential of this combination to improve PDT outcome by increasing cell toxicity. Many techniques were used to verify the combined effect. Photobiomodulation (PBM) enhanced PDT action in SCC-25 cells by increasing photosensitizer (PS) uptake and production of reactive oxygen species (ROS). The equivalent was not seen in SCC-4 cells compared to the PDT only group. We believe these effects are strongly related to the interval of application between PBMT, PS incubation and PDT. Additionally, the effect of ascorbic acid on preventing PBM effects in PDT shows that ROS play an important role in the early mechanisms of PBM-PDT. Therefore, we believe PBM-PDT combination is worth exploring, for its benefit-cost ratio and simple protocols, along with the possibility of improvement in treatment resuts. (AU)

FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 17/14182-4 - Development of a technique combining photobiomodulation and radiotherapy to enhance the tumoral response to ionizing radiation
Grantee:Clara Maria Gonçalves de Faria
Support Opportunities: Scholarships in Brazil - Doctorate