| Full text | |
| Author(s): Show less - |
Barros, Natan R.
[1, 2, 3, 4]
;
Kim, Han-Jun
[2, 4, 5]
;
Gouidie, Marcus J.
[2, 5]
;
Lee, KangJu
[2, 4, 5]
;
Bandaru, Praveen
[2, 4, 5]
;
Banton, Ethan A.
[2, 5]
;
Sarikhani, Einollah
[2, 5]
;
Sun, Wujin
[2, 4, 5]
;
Zhang, Shiming
[2, 5, 6]
;
Cho, Hyun-Jong
[2, 5, 7]
;
Hartel, Martin C.
[2, 5]
;
Ostrovidov, Serge
[2, 5]
;
Ahadian, Samad
[2, 4, 5]
;
Hussain, Saber M.
[8]
;
Ashammakhi, Nureddin
[2, 5, 9, 10]
;
Dokmeci, Mehmet R.
[2, 4, 5, 9]
;
Herculano, Rondinelli D.
[1]
;
Lee, Junmin
[2, 4, 5]
;
Khademhosseini, Ali
[2, 4, 5, 11, 12]
Total Authors: 19
|
| Affiliation: Show less - | [1] Sao Paulo State Univ Unesp, Inst Chem, 55 Prof Francisco Degni St, BR-14800060 Araraquara, SP - Brazil
[2] Univ Calif Los Angeles, Ctr Minimally Invas Therapeut C MIT, Los Angeles, CA 90095 - USA
[3] Saao Paulo State Univ Unesp, Sch Pharmaceut Sci, Bioproc & Biotechnol Dept, Km 01 Araraquara Jau Rd, BR-14801902 Araraquara, SP - Brazil
[4] Terasaki Inst Biomed Innovat, Los Angeles, CA 90064 - USA
[5] Univ Calif Los Angeles, Dept Bioengn, Henry Samueli Sch Engn & Appl Sci, Los Angeles, CA 90095 - USA
[6] Univ Hong Kong, Dept Elect & Elect Engn, Hong Kong 999077 - Peoples R China
[7] Kangwon Natl Univ, Coll Pharm, Chunchon 24341, Gangwon - South Korea
[8] US Air Force, Res Lab 711th Human Performance Wing, Airman Syst Directorate, Bioeffects Div, Mol Bioeffects Branch USAFRL 711HP, Wright Patterson AFB, OH 45433 - USA
[9] Univ Calif Los Angeles, Dept Radiol Sci, Los Angeles, CA 90095 - USA
[10] Michigan State Univ, Dept Biomed Engn, E Lansing, MI 48824 - USA
[11] Univ Calif Los Angeles, Henry Samueli Sch Engn & Appl Sci, Dept Chem & Biomol Engn, Los Angeles, CA 90095 - USA
[12] Univ Calif Los Angeles, Jonsson Comprehens Canc Ctr, Los Angeles, CA 90095 - USA
Total Affiliations: 12
|
| Document type: | Journal article |
| Source: | BIOFABRICATION; v. 13, n. 3 JUL 2021. |
| Web of Science Citations: | 2 |
| Abstract | |
The skin serves a substantial number of physiological purposes and is exposed to numerous biological and chemical agents owing to its large surface area and accessibility. Yet, current skin models are limited in emulating the multifaceted functions of skin tissues due to a lack of effort on the optimization of biomaterials and techniques at different skin layers for building skin frameworks. Here, we use biomaterial-based approaches and bioengineered techniques to develop a 3D skin model with layers of endothelial cell networks, dermal fibroblasts, and multilayered keratinocytes. Analysis of mechanical properties of gelatin methacryloyl (GelMA)-based bioinks mixed with different portions of alginate revealed bioprinted endothelium could be better modeled to optimize endothelial cell viability with a mixture of 7.5% GelMA and 2% alginate. Matrix stiffness plays a crucial role in modulating produced levels of Pro-Collagen I alpha-1 and matrix metalloproteinase-1 in human dermal fibroblasts and affecting their viability, proliferation, and spreading. Moreover, seeding human keratinocytes with gelatin-coating multiple times proved to be helpful in reducing culture time to create multiple layers of keratinocytes while maintaining their viability. The ability to fabricate selected biomaterials for each layer of skin tissues has implications in the biofabrication of skin systems for regenerative medicine and disease modeling. (AU) | |
| FAPESP's process: | 18/11861-0 - Skin-on-a-chip model for drug treatment study |
| Grantee: | Natan Roberto de Barros |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |