Using the Cecal Ligation and Puncture Model of Sepsis to Induce Rats to Multiple Organ Dysfunction

Author(s)

Condor Capcha, Jose Manuel ^{[1, 2]} ; Moreira, Roberto S. ^[3] ; Rodrigues, Camila E. ^[1] ; Silveira, Marcelo A. D. ^[1] ; Andrade, Lucia ^[1] ; Gomes, Samirah A. ^[2]

Affiliation

^[1] Univ Sao Paulo, Sch Med, Lab Basic Res, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Med, Lab Genet Cellular Biol & Mol Biol, Sao Paulo - Brazil

^[3] Fed Univ Catalao, Catalao - Brazil

• Document type: Journal article
• Source: BIO-PROTOCOL; v. 11, n. 7 APR 5 2021.
• Web of Science Citations: 0

Abstract

Sepsis is a dysregulated hyperinflammatory disease caused by infection. Sepsis leads to multiple organ dysfunction syndrome (MODS), which is associated with high rates of mortality. The cecal ligation and puncture (CLP) model has been widely used in animals and has become the gold-standard method of replicating features of sepsis in humans. Despite several studies and modified CLP protocols, there are still open questions regarding the multifactorial determinants of its reproducibility and medical significance. In our protocol, which is also aimed at mimicking the sepsis observed in clinical practice, male Wistar rats are submitted to CLP with adequate fluid resuscitation (0.15 M NaCl, 25 ml/kg BW i.p.) immediately after surgery. At 6 h after CLP, additional fluid therapy (0.15 M NaCl, 25 ml/kg BW s.c.) and antibiotic therapy with imipenem-cilastatin (single dose of 14 mg/kg BW s.c.) are administered. The timing of the fluid and antibiotic therapy correspond to the initial care given when patients are admitted to the intensive care unit. This model of sepsis provides a useful platform for simulating human sepsis and could lay the groundwork for the development of new treatments. (AU)

• FAPESP's process: 15/21308-9 - Mesenchymal stem cells derived from Wharton's Jelly in cardiopulmonary injury and systemic neuroimmunomodulation in sepsis
• Grantee: José Manuel Cóndor Capcha
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 10/19012-0 - Evaluation of hematopoietic stem cell treatment in dogs with chronic renal failure
• Grantee: Lucia da Conceição Andrade
• Support Opportunities: Research Projects - Thematic Grants