| Full text | |
| Author(s): |
Borelli, Tiago Cabral
[1]
;
Lovate, Gabriel Lencioni
[1]
;
Scaranello, Ana Flavia Tonelli
[1]
;
Ribeiro, Lucas Ferreira
[1]
;
Zaramela, Livia
[2]
;
Pereira-dos-Santos, Felipe Marcelo
[3]
;
Silva-Rocha, Rafael
[3]
;
Guazzaroni, Maria-Eugenia
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Biol, Ave Bandeirantes 3900, BR-14049901 Ribeirao Preto, SP - Brazil
[2] Univ Calif San Diego, Dept Pediat, La Jolla, CA 92161 - USA
[3] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Cell & Mol Biol, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | ANTIBIOTICS-BASEL; v. 10, n. 4 APR 2021. |
| Web of Science Citations: | 0 |
| Abstract | |
(1) Background: The rise of multi-antibiotic resistant bacteria represents an emergent threat to human health. Here, we investigate antibiotic resistance mechanisms in bacteria of several species isolated from an intensive care unit in Brazil. (2) Methods: We used whole-genome analysis to identify antibiotic resistance genes (ARGs) and plasmids in 34 strains of Gram-negative and Gram-positive bacteria, providing the first genomic description of Morganella morganii and Ralstonia mannitolilytica clinical isolates from South America. (3) Results: We identified a high abundance of beta-lactamase genes in resistant organisms, including seven extended-spectrum beta-lactamases (OXA-1, OXA-10, CTX-M-1, KPC, TEM, HYDRO, BLP) shared between organisms from different species. Additionally, we identified several ARG-carrying plasmids indicating the potential for a fast transmission of resistance mechanism between bacterial strains. Furthermore, we uncovered two pairs of (near) identical plasmids exhibiting multi-drug resistance. Finally, since many highly resistant strains carry several different ARGs, we used functional genomics to investigate which of them were indeed functional. In this sense, for three bacterial strains (Escherichia coli, Klebsiella pneumoniae, and M. morganii), we identified six beta-lactamase genes out of 15 predicted in silico as those mainly responsible for the resistance mechanisms observed, corroborating the existence of redundant resistance mechanisms in these organisms. (4) Conclusions: Systematic studies similar to the one presented here should help to prevent outbreaks of novel multidrug-resistant bacteria in healthcare facilities. (AU) | |
| FAPESP's process: | 19/00390-0 - Resistome and mobilome in genomes of bacteria from diverse Brazilian regions |
| Grantee: | Tiago Cabral Borelli |
| Support Opportunities: | Scholarships in Brazil - Master |
| FAPESP's process: | 15/04309-1 - Novel approaches to improve functional screening of biocatalysts in metagenomic libraries |
| Grantee: | María Eugenia Guazzaroni |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 19/06672-7 - Genome assembly for identification of antibiotic resistance genes in clinical isolates of the Hospital das Clínicas de Ribeirão Preto |
| Grantee: | Murilo Henrique Anzolini Cassiano |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 16/18827-7 - Integrating protein engineering and synthetic biology to develop new high-throughput selection systems |
| Grantee: | Lucas Ferreira Ribeiro |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 19/15675-0 - Unravelling the complexity of microbial gene regulatory networks |
| Grantee: | Rafael Silva Rocha |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 18/18158-3 - Search for determinants of antibiotic resistance in the interior of São Paulo using functional metagenomics |
| Grantee: | Gabriel Lencioni Lovate |
| Support Opportunities: | Scholarships in Brazil - Master |