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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Exploring G protein-coupled receptors and yeast surface display strategies for viral detection in baker's yeast: SARS-CoV-2 as a case study

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Author(s):
Maneira, Carla [1] ; Bermejo, Pamela Magali [2] ; Guimaraes Pereira, Goncalo Amarante [1] ; Bezerra de Mello, Fellipe da Silveira [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Campinas, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Rua Monteiro Lobato 255, BR-13083862 Campinas - Brazil
[2] Univ Estadual Campinas, Sch Food Engn, Rua Monteiro Lobato 80, BR-13083862 Campinas - Brazil
Total Affiliations: 2
Document type: Review article
Source: FEMS Yeast Research; v. 21, n. 1 FEB 2021.
Web of Science Citations: 0
Abstract

Viral infections pose intense burdens to healthcare systems and global economies. The correct diagnosis of viral diseases represents a crucial step towards effective treatments and control. Biosensors have been successfully implemented as accessible and accurate detection tests for some of the most important viruses. While most biosensors are based on physical or chemical interactions of cell-free components, the complexity of living microorganisms holds a poorly explored potential for viral detection in the face of the advances of synthetic biology. Indeed, cell-based biosensors have been praised for their versatility and economic attractiveness, however, yeast platforms for viral disease diagnostics are still limited to indirect antibody recognition. Here we propose a novel strategy for viral detection in Saccharomyces cereuisiae, which combines the transductive properties of G Protein-Coupled Receptors (GPCRs) with the Yeast Surface Display (YSD) of specific enzymes enrolled in the viral recognition process. The GPCR/YSD complex might allow for active virus detection through a modulated signal activated by a GPCR agonist, whose concentration correlates to the viral titer. Additionally, we explore this methodology in a case study for the detection of highly pathogenic coronaviruses that share the same cell receptor upon infection (i.e. the Angiotensin-Converting Enzyme 2, ACE2), as a conceptual example of the potential of the GPCR/YSD strategy for the diagnosis of COVID-19. (AU)

FAPESP's process: 18/03403-2 - Matabolic engineering of Saccharomyces cerevisiae wild strains using the CRISPR/Cas9 system
Grantee:Carla Maneira da Silva
Support Opportunities: Scholarships in Brazil - Master