Consequences of the exposure to bisphenol A in cell membrane models at the molecular level and hamster ovary cells viability

Author(s)

Maximino, Mateus D. ^[1] ; Silva, Carla Y. ^[1] ; Cavalcante, Dalita G. S. M. ^[1] ; Martin, Cibely S. ^[1] ; Job, Aldo E. ^[1] ; Oliveira, Jr., Osvaldo N. ^[2] ; Alessio, Priscila ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Technol & Appl Sci, BR-19060080 Presidente Prudente, SP - Brazil

^[2] Univ Sao Paulo, Sao Carlos Inst Phys, CP 369, BR-13560970 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 203, JUL 2021.
• Web of Science Citations: 1

Abstract

The inadequate disposal and the difficulty in its removal from water treatment systems have made the endocrine disruptor bisphenol A (BPA) a significant hazard for humans and animals. The molecular-level mechanisms of BPA action are not known in detail, which calls for systematic investigations using cell membrane models. This paper shows that BPA affects Langmuir monolayers and giant unilamellar vesicles (GUVs) of 1,2-dipalmitoyl-snglycero-3-phosphocholine (DPPC) used as membrane models, in a concentration-dependent manner and with effects that depend on BPA aggregation. BPA increases DPPC monolayer fluidity in surface pressure isotherms upon interacting with the headgroups through hydrogen bonding, according to polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS). In DPPC GUVs, BPA induced wrinkling and distortion in the spherical shape of the vesicles, but this was only observed for fresh solutions where it is not aggregated. BPA also decreased the viability of hamster ovary cells (CHO) in in vitro experiments. In contrast, aged, aggregated BPA solutions did not affect the GUVs and even increased CHO viability. These results may be rationalized in terms of size-dependent effects of BPA, which may be relevant for its endocrine-disrupting effects. (AU)

• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/06534-8 - Impact of Emerging Pollutants in Health: a Study on Biomimetic Membranes
• Grantee: Priscila Alessio Constantino
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/15019-0 - Combination of confocal fluorescence microscopy and surface-enhanced Raman scattering techniques: interaction, detection, and quantification of molecules of interest in biomimetic system.
• Grantee: Cibely da Silva Martin Sonvesso
• Support Opportunities: Scholarships in Brazil - Post-Doctoral