Evaluation of cytotoxic, genotoxic and mutagenic effects of titanium dioxide (TiO2) nanoparticles on CHO-K1 cell line derived from Chinese hamster ovary

• Grant number: 17/19600-9
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: January 01, 2018
• End date: December 31, 2018
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Marisa Narciso Fernandes
• Grantee: Cibele Bonicelli Gambarotto
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 14/05701-0 - Integrated responses of curimbatá, Prochilodus lineatus (Teleostei) to titanium dioxide (TiO2) nanoparticles and biomarkers identification for environmental monitoring, AP.TEM

Abstract

Due to their diminutive size, nanomaterials have large surface area and greater reactivity because of the high level of energy contained in the surface of the atoms. Titanium dioxide nanoparticles (TiO2-NP) are employed in numerous consumer products, for example, food, cosmetics and pharmaceuticals, the intensive use of TiO2-NP has been associated with lung, kidney and liver diseases, including gene mutations. Due to wide application, it is necessary to study the possible effects of TiO2-NP on cells. The aim of this study was to evaluate the cell viability and the genotoxic and mutagenic effects of different concentrations (0.25, 0.5, 0.75, 1.0 ¼g mL-1) of TiO2-NP in Chinese hamster ovary cells (CHO-k1) after 24 h exposure. TiO2-NP will be characterized relating zeta potential and hydrodynamic diameter in culture medium with and without fetal bovine serum. Cell viability will be assessed using the trypan blue dye exclusion assay and the genotoxic and mutagenic effects will be evaluated using the comet assay and micronucleus test, respectively. (AU)