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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Diagnostic Accuracy of HemotypeSC as a Point-of-Care Testing Device for Sickle Cell Disease: Findings from a Southwestern State in Nigeria and Implications for Patient Care in Resource-Poor Settings of sub-Saharan Africa

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Author(s):
Olatunya, Oladele S. [1, 2] ; Albuquerque, Dulcinea M. [1] ; Fagbamigbe, Adeniyi F. [3] ; Faboya, Opeyemi A. [2] ; Ajibola, Ayotunde E. [4] ; Babalola, Oluwatoyin A. [3] ; Adebisi, Adewale O. [4] ; Falusi, Adeyinka G. [3] ; Adekile, Adekunle [5] ; Costa, Fernando F. [1]
Total Authors: 10
Affiliation:
[1] Univ Estadual Campinas, Campinas - Brazil
[2] Ekiti State Univ, Ado Ekiti, Ekiti State - Nigeria
[3] Univ Ibadan, Ibadan, Oyo - Nigeria
[4] Ekiti State Univ, Teaching Hosp, Ado Ekiti, Ekiti State - Nigeria
[5] Kuwait Univ, Kuwait - Kuwait
Total Affiliations: 5
Document type: Journal article
Source: GLOBAL PEDIATRIC HEALTH; v. 8, MAY 2021.
Web of Science Citations: 0
Abstract

This study aimed to determine the performance of a rapid, point-of-care testing device (HemotypeSC)(TM) for diagnosing sickle cell disease (SCD) relative to 2 commonly-used methods compared to DNA polymerase chain reaction (PCR) as the reference standard. The diagnostic performance of (HemotypeSC)(TM) in diagnosing SCD and determining various other Hb genotypes relative to high performance liquid chromatography (HPLC) and cellulose acetate Hb electrophoresis in alkaline buffer (CAE) was investigated among 156 participants aged 4 to 23 years in Ekiti, Southwest Nigeria. PCR was considered as the reference method/gold standard. The sensitivity and specificity for SS, SC, AS, AC, and AA genotypes by HemotypeSC and HPLC when compared with PCR, were each 100%. Similarly, their positive and negative predictive values were each 100%. However, sensitivity and specificity for identifying these Hb genotypes by CAE were 100, 100, 96.5, 0, 99.2%, and 99, 100, 92.9, 0, 91.7%. Also, CAE did not identify any of the 2 HbAC individuals that were correctly identified by PCR and both HemotypeSC, and HPLC, thus representing 100% HbAC misdiagnosis. In conclusion, this study shows that HemotypeSC has perfect concordance with PCR and 100% accuracy in diagnosing SCD in the population tested. Its ease of use, accuracy and other attributes make it suitable for use in sub-Saharan Africa for rapid determination of Hb genotypes. (AU)

FAPESP's process: 14/00984-3 - Red blood cell disorders: pathophysiology and new therapeutic approaches
Grantee:Fernando Ferreira Costa
Support Opportunities: Research Projects - Thematic Grants