Recent 1D and 2D TD-NMR Pulse Sequences for Plant Science

Author(s)

Monaretto, Tatiana ^{[1, 2]} ; Moraes, Tiago Bueno ^[3] ; Colnago, Luiz Alberto ^[1]

Affiliation

^[1] Embrapa Instrumentacao, Rua 15 Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil

^[2] Univ Sao Paulo, Inst Quim Sao Carlos, Av Trabalhador Sao Carlense 400, BR-13566590 Sao Carlos, SP - Brazil

^[3] Univ Fed Minas Gerais, Dept Quim, Inst Ciencias Exatas, Av Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG - Brazil

• Document type: Review article
• Source: PLANTS-BASEL; v. 10, n. 5 MAY 2021.
• Web of Science Citations: 0

Abstract

Time domain nuclear magnetic resonance (TD-NMR) has been widely applied in plant science in the last four decades. Several TD-NMR instruments and methods have been developed for laboratory, green-house, and field studies. This mini-review focuses on the recent TD-NMR pulse sequences applied in plant science. One of the sequences measures the transverse relaxation time (T-2) with minimal sample heating, using a lower refocusing flip angle and consequently lower specific absorption rate than that of conventional CPMG. Other sequences are based on a continuous wave free precession (CWFP) regime used to enhance the signal-to-noise ratio, to measure longitudinal (T-1) and transverse relaxation time in a single shot experiment, and as alternative 2D pulse sequences to obtain T-1-T-2 and diffusion-T-1 correlation maps. This review also presents some applications of these sequences in plant science. (AU)

• FAPESP's process: 19/13656-8 - Development and application of nuclear magnetic resonance in products and agricultural processes
• Grantee: Luiz Alberto Colnago
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/07017-0 - Development of non-destructive methods for assessing the quality of intact food by NMR in the time domain
• Grantee: Tatiana Monaretto
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training