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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hormonal and neural responses to restraint stress in an animal model of perimenopause in female rats

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Author(s):
Arikawe, Adesina Paul [1, 2] ; Rorato, Rodrigo Cesar [3, 4] ; Gomes, Nathali [3] ; Elias, Lucila Leico [3] ; Anselmo-Franci, Janete [1]
Total Authors: 5
Affiliation:
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Basic & Oral Biol Pathol, Lab Neuroendocrinol, Sao Paulo - Brazil
[2] Univ Lagos, Coll Med, Fac Basic Med Sci, Dept Physiol, Block F, Room 024, Lagos 100254 - Nigeria
[3] Univ Sao Paulo, Sch Med Ribeirao Preto, Dept Physiol, Sao Paulo - Brazil
[4] Univ Fed Sao Paulo, Dept Biofis, Escola Paulista Med, Sao Paulo - Brazil
Total Affiliations: 4
Document type: Journal article
Source: Journal of Neuroendocrinology; v. 33, n. 5 MAY 2021.
Web of Science Citations: 0
Abstract

The present study investigated the hormonal and neural responses to stress in a perimenopause animal model induced by 4-vinylcyclohexene diepoxide (VCD), which induces progressive follicular depletion in rodents, allowing studies on the transition to ovarian failure. Female rats, aged 28 days old, were s.c. injected for 15 consecutive days with corn oil or VCD. At 85 +/- 5 days after the onset of treatment, the jugular vein was cannulated in the afternoon of metoestrus and in next morning (dioestrus) at 10.00 am, rats were subjected to 30 minutes of restraint stress. Blood samples were withdrawn before (-5 minutes), during (2, 5, 15 and 30 minutes) and after (45, 60 and 90 minutes) stress and plasma prolactin, progesterone and corticosterone levels were measured. Animals were perfused, brains processed for c-Fos/tyrosine hydroxylase (TH) in the locus coeruleus (LC) and c-Fos/corticotrophin-releasing factor (CRF) in the paraventricular nucleus (PVN). In unstressed rats the density of beta-endorphin fibres was assessed in LC and PVN. In VCD-treated rats, stress-induced prolactin peak was higher, basal and peak progesterone levels were lower, and both levels of corticosterone were similar to controls. However, the recovery period was longer for both adrenal hormones. In VCD-treated rats the number of c-Fos/TH and c-Fos/CRF-immunoreactive neurones was higher whereas the density of beta-endorphin fibres was lower in LC and PVN. We surmise that the hyperactivity of the LC and PVN neurones in VCD-treated rats may be a result of the lower progesterone levels that resulted in the decrease of beta-endorphin content in both nuclei, thus impairing the negative-feedback mechanism in the recovery period. (AU)

FAPESP's process: 12/51176-9 - Hormonal profile and stress responses of perimenopause animal model
Grantee:Arikawe Adesina Paul
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/09772-9 - Hormonal profile, response to stress and anxiety assessment in an animal model of perimenopause.
Grantee:Janete Aparecida Anselmo Franci
Support Opportunities: Regular Research Grants