Polymorphisms in xenobiotic metabolism-related genes in patients with hepatocellular carcinoma: a case-control study

Author(s):Show less -

Ferreira, Gislaine Dionisio ^[1] ; de Mendonca Fernandes, Glaucia Maria ^[1] ; Penteado, Camila ^[1] ; Coria, Vivian Romanholi ^[1] ; da Silva Galbiatti-Dias, Ana Livia ^{[1, 2]} ; Russo, Anelise ^[1] ; Urbanin Castanhole-Nunes, Marcia Maria ^{[1, 2]} ; da Silva, Renato Ferreira ^{[2, 3]} ; Martins Alves da Silva, Rita de Cassia ^{[2, 3]} ; Pavarino, Erika Cristina ^{[1, 2]} ; Ruiz Cintra, Mariangela Torreglosa ^[4] ; Goloni-Bertollo, Eny Maria ^{[1, 2]}

Affiliation

^[1] Sao Jose do Rio Preto Med Sch FAMERP, Mol Biol Dept, Genet & Mol Biol Res Unit UPGEM, Av Brigadeiro Faria Lima 5416, BR-15090000 Sao Jose Do Rio Preto, SP - Brazil

^[2] Sao Jose do Rio Preto Med Sch Fdn FUNFARME, Sao Jose Do Rio Preto - Brazil

^[3] Sao Jose do Rio Preto Med Sch Fdn FAMERP FUNFARME, Surg Dept, Study Grp Liver Tumors GETF, Sao Jose Do Rio Preto - Brazil

^[4] Fed Univ Triangulo Mineiro UFTM, Dept Biol Sci DCB, Uberaba - Brazil

• Document type: Journal article
• Source: XENOBIOTICA; v. 51, n. 6, p. 737-744, JUN 3 2021.
• Web of Science Citations: 0

Abstract

This study was performed to investigate the relationship between polymorphisms in microsomal epoxide hydrolase (mEH; Tyr113His and His139Arg substitution) and glutathione S-transferase (GST; GSTM1 deletion, GSTT1 deletion, and GSTP1.Ala114Val substitution) and their correlation with clinico-histopathological features in hepatocellular carcinoma (HCC). We evaluated environmental risk factors and genetic alterations in 556 individuals (86 cases and 470 controls). PCR multiplex for GSTM1 and GSTT1, polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) for GSTP1, and real-time PCR for mEH were performed. Statistical analyses were performed using multiple logistic regression tests. Age over 48 years (p < 0.001) and alcohol consumption (p = 0.021) were the predictors of increased risk of developing HCC. GSTP1.Ala114Val for all regression models (p < 0.05), except the recessive model, and the GSTT1 null genotype (odds ratio {[}OR] = 0.43, 95% confidence interval {[}CI] = 0.21-0.87, p = 0.019) were predictors of an increased risk of developing HCC. Polymorphic GSTT1, GSTM1, GSTP1.Ala114Val, and mEH.His139Arg and wild-type mEH.Tyr113His (OR = 5.04; 95% CI = 1.59-16.04; p = 0.006) were associated with HCC. Age over 48 years, alcohol consumption, and the presence of polymorphic variants of GSTP1 and GSTT1 were associated with the risk of developing HCC. (AU)

• FAPESP's process: 12/14781-1 - Evaluation of polymorphisms of xenobiotic metabolizing genes (GSTM1, GSTT1, GSTP1 e mEH) in patients with cirrhosis and hepatocellular carcinoma
• Grantee: Eny Maria Goloni Bertollo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/02473-0 - Epidemiological and molecular investigation of genes related to xenobiotic metabolism in patients with sporadic colerrectal cancer
• Grantee: Glaucia Maria de Mendonça Fernandes
• Support Opportunities: Scholarships in Brazil - Master