| Full text | |
| Author(s): Show less - |
Clark, Iain C.
[1, 2]
;
Gutierrez-Vazquez, Cristina
[1]
;
Wheeler, Michael A.
[3, 1]
;
Li, Zhaorong
[3, 1]
;
Rothhammer, Veit
[1, 4]
;
Linnerbauer, Mathias
[1, 4]
;
Sanmarco, Liliana M.
[1]
;
Guo, Lydia
[1]
;
Blain, Manon
[5]
;
Zandee, Stephanie E. J.
[6]
;
Chao, Chun-Cheih
[1]
;
Batterman, V, Katelyn
;
Schwabenland, Marius
[7]
;
Lotfy, Peter
[3, 1]
;
Tejeda-Velarde, Amalia
[8, 1, 9]
;
Hewson, Patrick
[1]
;
Polonio, Carolina Manganeli
[1]
;
Shultis, Michael W.
[1]
;
Salem, Yasmin
[1]
;
Tjon, Emily C.
[1]
;
Fonseca-Castro, Pedro H.
[1]
;
Borucki, Davis M.
[1]
;
de Lima, Kalil Alves
[1]
;
Plasencia, Agustin
[1]
;
Abate, Adam R.
[10, 11]
;
Rosene, Douglas L.
[12]
;
Hodgetts, Kevin J.
[1]
;
Prinz, Marco
[7, 13, 14, 15]
;
Antel, Jack P.
[5]
;
Prat, Alexandre
[6]
;
Quintana, Francisco J.
[3, 1]
Total Authors: 31
|
| Affiliation: Show less - | [1] Harvard Med Sch, Ann Romney Ctr Neurol Dis, Brigham & Womens Hosp, Boston, MA 02115 - USA
[2] Univ Calif Berkeley, Calif Inst Quantitat Biosci, Dept Bioengn, Berkeley, CA 94720 - USA
[3] Broad Inst MIT & Harvard, Cambridge, MA 02142 - USA
[4] Friedrich Alexander Univ Erlangen Nurnberg, Univ Hosp Erlangen, Dept Neurol, D-91054 Erlangen - Germany
[5] McGill Univ, Montreal Neurol Inst, Dept Neurol & Neurosurg, Neuroimmunol Unit, Montreal, PQ H3A 2B4 - Canada
[6] Univ Montreal CRCHUM, Neuroimmunol Res Lab, Ctr Rech, Ctr Hosp, Montreal, PQ H2X 0A9 - Canada
[7] Univ Freiburg, Inst Neuropathol, D-79106 Freiburg - Germany
[8] Inst Ramon y Cajal Invest Sanitaria IRYCIS, Madrid - Spain
[9] Hosp Univ Ramon y Cajal, Immunol Dept, Madrid - Spain
[10] Univ Calif San Francisco, Calif Inst Quantitat Biosci, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 - USA
[11] Chan Zuckerberg Biohub, San Francisco, CA - USA
[12] Batterman, Katelyn, V, Boston Univ, Sch Med, Dept Anat & Neurobiol, Boston, MA 02118 - USA
[13] Univ Freiburg, Signaling Res Ctr BIOSS, D-79106 Freiburg - Germany
[14] Univ Freiburg, Signaling Res Ctr CIBSS, D-79106 Freiburg - Germany
[15] Univ Freiburg, Fac Med, Ctr Basics NeuroModulat NeuroModulBas, D-79106 Freiburg - Germany
Total Affiliations: 15
|
| Document type: | Journal article |
| Source: | Science; v. 372, n. 6540, p. 360+, APR 23 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Cell-cell interactions control the physiology and pathology of the central nervous system (CNS). To study astrocyte cell interactions in vivo, we developed rabies barcode interaction detection followed by sequencing (RABID-seq), which combines barcoded viral tracing and single-cell RNA sequencing (scRNA-seq). Using RABID-seq, we identified axon guidance molecules as candidate mediators of microgliaastrocyte interactions that promote CNS pathology in experimental autoimmune encephalomyelitis (EAE) and, potentially, multiple sclerosis (MS). In vivo cell- specific genetic perturbation EAE studies, in vitro systems, and the analysis of MS scRNA-seq datasets and CNS tissue established that Sema4D and Ephrin-B3 expressed in microglia control astrocyte responses via PlexinB2 and EphB3, respectively. Furthermore, a CNS-penetrant EphB3 inhibitor suppressed astrocyte and microglia proinflammatory responses and ameliorated EAE. In summary, RABID-seq identified microgliaastrocyte interactions and candidate therapeutic targets. (AU) | |
| FAPESP's process: | 19/13731-0 - MicroRNAs, neurodevelopment and antiviral immune response: how they can be connected? |
| Grantee: | Carolina Manganeli Polonio |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |