An Actinobacterium Strain From Soil of Cerrado Promotes Phosphorus Solubilization and Plant Growth in Soybean Plants

Author(s)

Vargas Hoyos, Harold Alexander ^{[1, 2]} ; Chiaramonte, Josiane Barros ^[2] ; Barbosa-Casteliani, Ana Gabriele ^[2] ; Fernandez Morais, Jorge ^[2] ; Perez-Jaramillo, Juan Esteban ^[1] ; Nobre Santos, Suikinai ^[2] ; Nascimento Queiroz, Sonia Claudia ^[2] ; Soares Melo, Itamar ^[2]

Affiliation

^[1] Univ Antioquia, Sch Med, Program Study & Control Trop Dis PECET, Medellin - Colombia

^[2] Embrapa Environm, Lab Environm Microbiol, Jaguariuna - Brazil

• Document type: Journal article
• Source: FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY; v. 9, APR 22 2021.
• Web of Science Citations: 0

Abstract

The huge biological diversity of the Brazilian Cerrado is an important source of economically interesting microbial agents. The phylum Actinobacteria plays an important role in nutrient cycling, potentially improving their availability to plants. In this study, we isolated an actinobacteria (strain 3AS4) from wheat rhizospheres of crops cultivated in the Cerrado biome. Strain 3AS4 was identified as belonging to the genus Streptomyces and had phosphorus mobilization ability, mineralizing approximately 410 mu g ml(-1) from phytate, 300 mu g ml(-1) from calcium phosphate, and 200 mu g ml(-1) from rock phosphate. The analysis of the actinobacteria crude extract by spectrometric techniques revealed the presence of gluconic and 2-ketogluconic acid, and a greenhouse experiment was carried out to evaluate its plant growth promotion activity in soybean. Soil in its natural condition (with no phosphorus addition), 40 kg ha(-1) rock phosphate from Bayovar (RP) added to soil, and triple super phosphate (SPT) added to soil were used. Significant differences in plant height were observed at 6 weeks when the plants were inoculated with the 3AS4 strain. The growth of inoculated plants in natural condition was promoted in 17% compared with the RP and SPT non-inoculated conditions, suggesting that inoculation can enable plants to grow with lower chemical P fertilizers. In the plants that were inoculated with the 3AS4 strain in the RP condition, the plant height increased by approximately 80% and the shoot:root ratio was approximately 30% higher compared to control conditions (non-inoculated plants in natural conditions). 3AS4 has P-solubilizing potential and can be exploited as an inoculant for soybean cultivation. These results suggest that this actinobacterium is a valuable resource for sustainable agriculture and will allow the reduction of phosphate fertilization in the future. (AU)

• FAPESP's process: 13/16037-0 - Isolation and characterization of genes correlated to biosynthesis of antitumor molecules in Streptomyces sp.
• Grantee: Suikinai Nobre Santos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 13/25076-0 - "Actinomycetes isolated from soils of Brazilian ecosystems: potential agents of biological control of Sclerotinia sclerotiorum in cultivation of soybean (Glycine max L. Merrill)"
• Grantee: Harold Alexander Vargas Hoyos
• Support Opportunities: Scholarships in Brazil - Doctorate