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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Overexpression of a Sugarcane BAHD Acyltransferase Alters Hydroxycinnamate Content in Maize Cell Wall

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Author(s):
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Fanelli, Amanda [1] ; Rancour, David M. [2] ; Sullivan, Michael [3] ; Karlen, Steven D. [4, 5] ; Ralph, John [4, 5] ; Riano-Pachon, Diego Mauricio [6] ; Vicentini, Renato [7] ; Silva, Tatiane da Franca [1] ; Ferraz, Andre [8] ; Hatfield, Ronald D. [3] ; Romanel, Elisson [1]
Total Authors: 11
Affiliation:
[1] Univ Sao Paulo, Escola Engn Lorena, Dept Biotecnol, Lab Genom Plantas & Bioenergia PGEMBL, Lorena - Brazil
[2] Lytic Solut LLC, Madison, WI - USA
[3] ARS, USDA, US Dairy Forage Res Ctr, Madison, WI - USA
[4] Univ Wisconsin, Wisconsin Energy Inst, Dept Biochem, Madison, WI - USA
[5] Univ Wisconsin, Wisconsin Energy Inst, Dept Energys Great Lakes Bioenergy Res Ctr, Madison, WI - USA
[6] Univ Sao Paulo, Ctr Energia Nucl Agr, Lab Biol Computac Evolutiva & Sistemas, Piracicaba - Brazil
[7] Univ Estadual Campinas, Inst Biol, Dept Genet & Evolucao, Campinas - Brazil
[8] Univ Sao Paulo, Escola Engenharia Lorena, Dept Biotecnol, Escola Engn Lorena, Lorena - Brazil
Total Affiliations: 8
Document type: Journal article
Source: FRONTIERS IN PLANT SCIENCE; v. 12, APR 21 2021.
Web of Science Citations: 2
Abstract

The purification of hydroxycinnamic acids {[}p-coumaric acid (pCA) and ferulic acid (FA)] from grass cell walls requires high-cost processes. Feedstocks with increased levels of one hydroxycinnamate in preference to the other are therefore highly desirable. We identified and conducted expression analysis for nine BAHD acyltransferase ScAts genes from sugarcane. The high conservation of AT10 proteins, together with their similar gene expression patterns, supported a similar role in distinct grasses. Overexpression of ScAT10 in maize resulted in up to 75% increase in total pCA content. Mild hydrolysis and derivatization followed by reductive cleavage (DFRC) analysis showed that pCA increase was restricted to the hemicellulosic portion of the cell wall. Furthermore, total FA content was reduced up to 88%, resulting in a 10-fold increase in the pCA/FA ratio. Thus, we functionally characterized a sugarcane gene involved in pCA content on hemicelluloses and generated a C4 plant that is promising for valorizing pCA production in biorefineries. (AU)

FAPESP's process: 14/17486-6 - Genomics of hemicellulose biosynthesis in sugarcane: understanding the relationship of genes and the recalcitrance of cell wall
Grantee:Elisson Antonio da Costa Romanel
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 16/24391-7 - Evolutionary and genomics functional analysis of sugarcane genes involved in the recalcitrance cell wall
Grantee:Elisson Antonio da Costa Romanel
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
FAPESP's process: 16/25785-9 - Prospection and biotechnological use evaluations of genes involved in the structural modification of crystalline cellulose in sugarcane
Grantee:Tatiane da Franca Silva
Support Opportunities: Regular Research Grants