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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Unraveling a Lignocellulose-Decomposing Bacterial Consortium from Soil Associated with Dry Sugarcane Straw by Genomic-Centered Metagenomics

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Author(s):
Weiss, Bruno [1, 2] ; Oliveira Souza, Anna Carolina [1, 2] ; Lima Constancio, Milena Tavares [1, 2] ; Alvarenga, Danillo Oliveira [2] ; Pylro, Victor S. [3] ; Alves, Lucia M. Carareto [2] ; Varani, Alessandro M. [2]
Total Authors: 7
Affiliation:
[1] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci, Grad Program Agr & Livestock Microbiol, BR-14884900 Jaboticabal, SP - Brazil
[2] Sao Paulo State Univ UNESP, Sch Agr & Vet Sci, Dept Technol, BR-14884900 Jaboticabal, SP - Brazil
[3] Fed Univ Lavras UFLA, Dept Biol, Microbial Ecol & Bioinformat Lab, BR-37200000 Lavras, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: MICROORGANISMS; v. 9, n. 5 MAY 2021.
Web of Science Citations: 0
Abstract

Second-generation biofuel production is in high demand, but lignocellulosic biomass' complexity impairs its use due to the vast diversity of enzymes necessary to execute the complete saccharification. In nature, lignocellulose can be rapidly deconstructed due to the division of biochemical labor effectuated in bacterial communities. Here, we analyzed the lignocellulolytic potential of a bacterial consortium obtained from soil and dry straw leftover from a sugarcane milling plant. This consortium was cultivated for 20 weeks in aerobic conditions using sugarcane bagasse as a sole carbon source. Scanning electron microscopy and chemical analyses registered modification of the sugarcane fiber's appearance and biochemical composition, indicating that this consortium can deconstruct cellulose and hemicellulose but no lignin. A total of 52 metagenome-assembled genomes from eight bacterial classes (Actinobacteria, Alphaproteobacteria, Bacilli, Bacteroidia, Cytophagia, Gammaproteobacteria, Oligoflexia, and Thermoleophilia) were recovered from the consortium, in which similar to 46% of species showed no relevant modification in their abundance during the 20 weeks of cultivation, suggesting a mostly stable consortium. Their CAZymes repertoire indicated that many of the most abundant species are known to deconstruct lignin (e.g., Chryseobacterium) and carry sequences related to hemicellulose and cellulose deconstruction (e.g., Chitinophaga, Niastella, Niabella, and Siphonobacter). Taken together, our results unraveled the bacterial diversity, enzymatic potential, and effectiveness of this lignocellulose-decomposing bacterial consortium. (AU)

FAPESP's process: 16/16624-1 - Deconstruction of lignocellulosic material by bacterial consortium
Grantee:Lúcia Maria Carareto Alves
Support Opportunities: Regular Research Grants
FAPESP's process: 10/17520-9 - Bacterial consortium for biomass degradation to produce ethanol
Grantee:Lúcia Maria Carareto Alves
Support Opportunities: Regular Research Grants