| Full text | |
| Author(s): Show less - |
Lima, Caroline Sprengel
[1]
;
Mottin, Melina
[2]
;
de Assis, Leticia Ribeiro
[1]
;
de Moraes Roso Mesquita, Nathalya Cristina
[3]
;
de Paula Sousa, Bruna Katiele
[2]
;
Coimbra, Lais Durco
[4]
;
Bispo-dos-Santos, Karina
[5]
;
Zorn, Kimberley M.
[6]
;
Guido, Rafael V. C.
[3]
;
Ekins, Sean
[6]
;
Marques, Rafael Elias
[4]
;
Proenca-Modena, Jose Luiz
[5]
;
Oliva, Glaucius
[3]
;
Andrade, Carolina Horta
[2]
;
Regasini, Luis Octavio
[1]
Total Authors: 15
|
| Affiliation: | [1] Sao Paulo State Univ Unesp, Inst Biosci Humanities & Exact Sci, Lab Antibiot & Chemotherapeut LAQ, Sao Jose Do Rio Preto, SP - Brazil
[2] Univ Fed Goias, Fac Farm, Lab Mol Modeling & Drug Design LabMol, Goiania, Go - Brazil
[3] Univ Sao Paulo, Inst Phys Sao Carlos, Sao Carlos, SP - Brazil
[4] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biosci Natl Lab LNBio, Campinas, SP - Brazil
[5] Univ Campinas UNICAMP, Inst Biol, Dept Genet Evolut Microbiol & Immunol, Lab Emerging Viruses LEVE, Campinas, SP - Brazil
[6] Collaborat Pharmaceut Inc, Raleigh, NC - USA
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | BIOORGANIC CHEMISTRY; v. 109, APR 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Although the widespread epidemic of Zika virus (ZIKV) and its neurological complications are well-known there are still no approved drugs available to treat this arboviral disease or vaccine to prevent the infection. Flavonoids from Pterogyne nitens have already demonstrated anti-flavivirus activity, although their target is unknown. In this study, we virtually screened an in-house database of 150 natural and semi-synthetic compounds against ZIKV NS2B-NS3 protease (NS2B-NS3p) using docking-based virtual screening, as part of the OpenZika project. As a result, we prioritized three flavonoids from P. nitens, quercetin, rutin and pedalitin, for experimental evaluation. We also used machine learning models, built with Assay Central (R) software, for predicting the activity and toxicity of these flavonoids. Biophysical and enzymatic assays generally agreed with the in silico predictions, confirming that the flavonoids inhibited ZIKV protease. The most promising hit, pedalitin, inhibited ZIKV NS2B-NS3p with an IC50 of 5 mu M. In cell-based assays, pedalitin displayed significant activity at 250 and 500 mu M, with slight toxicity in Vero cells. The results presented here demonstrate the potential of pedalitin as a candidate for hit-to-lead (H2L) optimization studies towards the discovery of antiviral drug candidates to treat ZIKV infections. (AU) | |
| FAPESP's process: | 16/00194-8 - Pathogenesis and neurovirulence of emerging viruses in Brazil |
| Grantee: | José Luiz Proença Módena |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 18/03917-6 - Mechanisms of disease susceptibility and resistance in experimental Mayaro fever in mice |
| Grantee: | Rafael Elias Marques Pereira Silva |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery |
| Grantee: | Glaucius Oliva |
| Support Opportunities: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |
| FAPESP's process: | 14/18330-0 - Synthesis and biological evaluation of curcumin-cinnamaldehyde hybrids as bacterial cell division inhibitors |
| Grantee: | Luis Octávio Regasini |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/15083-2 - Synthesis and biological evaluation of isobavachalcone (IBC) and analogs as potential agents against tuberculosis |
| Grantee: | Luis Octávio Regasini |
| Support Opportunities: | Regular Research Grants |