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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of vitamin D-induced supernatant of placental explants from preeclamptic women on oxidative stress and nitric oxide bioavailability in human umbilical vein endothelial cells

Full text
Author(s):
Nunes, P. R. [1] ; Gomes, V. J. [1] ; Sandrim, V. C. [2] ; Peracoli, J. C. [1] ; Peracoli, M. T. S. [1] ; Carlstrom, M. [3]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, Fac Med Botucatu, Dept Ginecol & Obstet, Botucatu, SP - Brazil
[2] Univ Estadual Paulista, Inst Biociencias, Dept Biofis & Farmacol, Botucatu, SP - Brazil
[3] Karolinska Inst, Dept Physiol & Pharmacol, Stockholm - Sweden
Total Affiliations: 3
Document type: Journal article
Source: Brazilian Journal of Medical and Biological Research; v. 54, n. 8 2021.
Web of Science Citations: 0
Abstract

The study evaluated the effect of the supernatant of placental explants from preeclamptic (PE) and normotensive (NT) pregnant women after tissue treatment with or without vitamin D (VD) on oxidative stress and nitric oxide (NO) bioavailability in human umbilical vein endothelial cells (HUVEC). Placental explants were prepared from eight NT and eight PE women, and supernatants were obtained after incubation with or without hydrogen peroxide (H2O2) and/or VD. HUVEC were cultured for 24 h with supernatants, and the following parameters were analyzed in HUVEC cultures: NO, nitrate (NO3-), and nitrite (NO2-) levels, lipid peroxidation, and intracellular reactive oxygen species (ROS). Results showed that the production of NO3-, NO2-, malondialdehyde (MDA), and ROS were significantly higher in HUVEC treated with explant supernatant from PE compared to NT pregnant women, while the supernatant of PE explants treated with VD led to a decrease in these parameters. A significantly high production of NO was detected in HUVEC cultured with control supernatant of NT group, and in cultures treated with supernatant of PE explants treated with VD. Taken together, these results demonstrated that cultures of placental explants from PE women with VD treatment generated a supernatant that decreased oxidative stress and increased the bioavailability of NO in endothelial cells. (AU)

FAPESP's process: 16/23452-2 - Immunomodulatory effect of vitamin D on the activation of inflammasomes in placental tissue from pregnant women with pre-eclampsia
Grantee:Priscila Rezeck Nunes
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 19/00234-8 - Effects of supernatant from placental explants treated with vitamin D on oxidative stress, inflammation and nitric oxide availability in endothelial cells
Grantee:Priscila Rezeck Nunes
Support Opportunities: Scholarships abroad - Research Internship - Doctorate