o-fermentation of sugarcane bagasse hydrolysate and molasses by Clostridium saccharoperbutylacetonicum: Effect on sugar consumption and butanol productio

Author(s)

Zetty-Arenas, Ana Maria ^{[1, 2, 3, 4]} ; Tovar, Laura Plazas ^[5] ; Alves, Rafael Ferraz ^{[2, 6]} ; Mariano, Adriano Pinto ^[1] ; van Gulik, Walter ^[3] ; Filho, Rubens Maciel ^[1] ; Freitas, Sindelia ^{[1, 7]}

Affiliation

^[1] Univ Estadual Campinas, Sch Chem Engn, BR-13083852 Campinas, SP - Brazil

^[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, BR-13083970 Campinas, SP - Brazil

^[3] Delft Univ Technol, Dept Biotechnol, NL-2629 HZ Delft - Netherlands

^[4] Univ Nacl Colombia, Inst Biotechnol & Agribusiness, Dept Chem Engn, Manizales 170004, Caldas - Colombia

^[5] Univ Fed Sao Paulo, Dept Chem Engn, BR-09913030 Diadema, SP - Brazil

^[6] Novozymes Amer Latina, BR-83707660 Araucaria, Para - Brazil

^[7] SENAI SP, Innovat Inst Biotechnol, BR-01311923 Sao Paulo - Brazil

• Document type: Journal article
• Source: INDUSTRIAL CROPS AND PRODUCTS; v. 167, SEP 1 2021.
• Web of Science Citations: 0

Abstract

Co-fermentation of mixed sugars to produce butanol is an attractive route in sucrochemical production chains. Herein, high-level mixed sugars from sugarcane bagasse hemicellulosic hydrolysate (HH) and molasses (SCM) were investigated as potential substrates for acetone-butanol-ethanol (ABE) production in batch fermentation by Clostridium saccharoperbutylacetonicum DSM 14923. HH produced after hydrothermal pretreatment was concentrated 5-fold and was called concentrated hemicellulosic hydrolysate (CHH). The fermentation media that were investigated consisted solely of CHH and SCM and three CHH-to-SCM ratios were used to provide 30 g/L initial sugar concentrations and furan derivatives lower than 0.1 g/L. For CHH50/SCM50 and CHH75/SCM25, diluting CHH to concentrations of 15 g/L sugars and 22.5 g/L sugars, respectively, which were supplemented with SCM and nutrients, suffered growth inhibition as a function of the concentration of undissociated acid in the medium. The best-performing medium, CHH25/SCM75 (7.5 g/L and 22.5 g/L sugars from CHH and SCM, respectively and about 0.017 g/L furan derivatives), showed 97 % sugar consumption, and in the pH range of 5.5-6.5, undissociated acetic acid was not an inhibitory molecular form to C. saccharoperbutylacetonicum. After 30 h of fermentation, 7.8 and 9.8 g/L butanol and ABE were produced, respectively, which yielded 0.28 and 0.36 g/g. Based on our findings, C. saccharoperbutylacetonicum exhibits its potential and effective application for renewable butanol production by co-fermenting mixed sugars. (AU)

• FAPESP's process: 15/20630-4 - Biorefinery development integrated to a bioethanol sugar cane plant with zero CO2 emission: routes to convert renewable resources to bio-products and bio-electricity
• Grantee: Rubens Maciel Filho
• Support Opportunities: Research Projects - Thematic Grants