Light-Emitting Probes for Labeling Peptides

Author(s)

Boaro, Andreia ^{[1, 2, 3]} ; Ageitos, Lucia ^{[2, 3, 4, 5]} ; Torres, Marcelo ^{[2, 3]} ; Bartoloni, Fernando Heering ^[1] ; de la Fuente-Nunez, Cesar ^{[2, 3]}

Affiliation

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Ave Estados 5001, BR-09210580 Santo Andre, SP - Brazil

^[2] Univ Penn, Perelman Sch Med, Inst Translat Med & Therapeut, Machine Biol Grp, Dept Psychiat & Microbiol, Inst B, 3610 Hamilton Walk, Philadelphia, PA 19104 - USA

^[3] Univ Penn, Dept Bioengn, 3610 Hamilton Walk, Philadelphia, PA 19104 - USA

^[4] Univ A Coruna, Fac Ciencias, Ctr Invest Cient Avanzadas CICA, Calle Maestranza 9, La Coruna 15071 - Spain

^[5] Univ A Coruna, Fac Ciencias, Dept Quim, Calle Maestranza 9, La Coruna 15071 - Spain

• Document type: Review article
• Source: CELL REPORTS PHYSICAL SCIENCE; v. 1, n. 12 DEC 23 2020.
• Web of Science Citations: 1

Abstract

Peptides are versatile biopolymers composed of 2-100 amino acid residues that present a wide range of biological functions and constitute potential therapies for numerous diseases, partly due to their ability to penetrate cell membranes, However, their mechanisms of action have not been fully elucidated due to the lack of appropriate tools. Existing light-emitting probes are limited by their cytotoxicity and large size, which can alter peptide structure and function. Here, we describe the available fluorescent, bioluminescent, and chemiluminescent probes for labeling peptides, with a focus on minimalistic options. (AU)

• FAPESP's process: 16/10585-4 - Mechanistic aspects regarding the induced decomposition of hydroperoxides and silylperoxides derived from lophine
• Grantee: Andréia Boaro
• Support type: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/15871-3 - Light emitting arginine derivatives as tags for antimicrobial peptides
• Grantee: Andréia Boaro
• Support type: Scholarships abroad - Research Internship - Doctorate