Taxonomic significance of the distribution of constituents of leaf cuticular waxes of Croton species (Euphorbiaceae)

Author(s)

Pimentel, Bruna Silvestroni ^[1] ; Negri, Giuseppina ^[1] ; Cordeiro, Ines ^[2] ; Motta, Lucimar Barbosa ^[3] ; Salatino, Antonio ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Bot, Rua Matao 277, BR-05508090 Sao Paulo, SP - Brazil

^[2] Inst Bot, Av Miguel Stefano 3687, BR-04301902 Sao Paulo, SP - Brazil

^[3] Univ Paulista UNIP, Av Prof Francisco Morato 3365, BR-05521000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Biochemical Systematics and Ecology; v. 92, OCT 2020.
• Web of Science Citations: 0

Abstract

Foliar epicuticular waxes of specimens of 13 Croton species native in Brazil were extracted. The fractions containing alkanes and primary alcohols were isolated by preparative thin layer chromatography. Derivatized n-primary alcohols were identified by gas chromatography (GC) coupled with mass spectrometry and n-alkanes by GC and comparison with known standards. Relative abundances were estimated by GC coupled with flame ionization detector. The distribution of constituents of both classes was analyzed by cluster analysis, using the UPGMA method and Euclidean distances. The chemical affinities among species were compared with published data of molecular phylogenetic relationships. The distribution of n-alkanes and primary alcohols were shown to be useful markers of Croton species. Primary alcohols were more consistent than n-alkanes for species fingerprinting. (AU)

• FAPESP's process: 08/10595-3 - Chemical diversity, genetic polymorphism and pharmacological activities of Croton (Euphorbiaceae)
• Grantee: Antonio Salatino
• Support Opportunities: Regular Research Grants