Polymeric biomaterials maintained the esthetic efficacy and reduced the cytotoxicity of in-office dental bleaching

Author(s)

Ortecho-Zuta, Uxua ^[1] ; de Oliveira Duque, Carla Caroline ^[1] ; de Oliveira Ribeiro, Rafael Antonio ^[1] ; Leite, Maria Luisa ^[1] ; Soares, Diana Gabriela ^[2] ; Hebling, Josimeri ^[3] ; Fraga Briso, Andre Luiz ^[4] ; Souza Costa, Carlos Alberto ^[5]

Affiliation

^[1] Sao Paulo State Univ UNESP, Sch Dent, Dept Dent Mat & Prosthodont, Araraquara, SP - Brazil

^[2] Sao Paulo Univ USP, Sch Dent, Dept Dent Endodont & Dent Mat, Bauru, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Morphol & Pediat Dent, Araraquara, SP - Brazil

^[4] Sao Paulo State Univ UNESP, Sch Dent, Dept Restorat Dent, Aracatuba - Brazil

^[5] Sao Paulo State Univ UNESP, Sch Dent, Dept Physiol & Pathol, Humaita St 1680, BR-14801385 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Journal of Esthetic and Restorative Dentistry; v. 33, n. 8 JUL 2021.
• Web of Science Citations: 0

Abstract

Evaluate the kinetics of hydrogen peroxide (H2O2) degradation, esthetic efficacy and cytotoxicity of a bleaching gel with 35%H2O2 applied on enamel previously covered or not with polymeric nanofibrillar scaffold (SNan), polymeric primer catalyst (PPol), and both. Standardized enamel/dentin discs (n = 128) obtained from bovine teeth were adapted to pulp chambers. After covering enamel with the polymeric products, the bleaching gel was applied for 45 min, establishing the following groups: G1: no treatment (negative control); G2: 35%H2O2 (positive control); G3: SNan; G4: PPol; G5: SNan + PPol; G6: SNan + 35%H2O2; G7: PPol + 35%H2O2; G8: SNan + PPol + 35%H2O2. The kinetics of H2O2 degradation (n = 8), bleaching efficacy (Delta E/Delta WI; n = 8), trans-amelodentinal cytotoxicity (n = 8), and cell morphology (n = 4) were assessed (ANOVA/Tukey test; p < 0.05). Greater H2O2 degradation occurred in G7 and G8. Bleaching efficacy (Delta E) was higher in G6, G7, and G8 in comparison with G2 (p < 0.05). However, no difference was observed for Delta WI (p > 0.05). G8 presented the lower level of trans-amelodentinal diffusion of H2O2, oxidative stress, and toxicity to the MDPC-23 cells (p < 0.05). Polymeric biomaterials increased the kinetics of H2O2 decomposition, as well as maintained the esthetic efficacy and minimized the cytotoxicity caused by a bleaching gel with 35%H2O2. Clinical Significance Application of a bleaching gel with 35%H2O2 on enamel previously covered by polymeric biomaterials maintains the esthetic efficacy and reduces the cytotoxicity caused by a single session of in-office dental bleaching. (AU)

• FAPESP's process: 17/22739-9 - Development and analysis of a bilayer scaffold for pulp-dentin regeneration
• Grantee: Carlos Alberto de Souza Costa
• Support Opportunities: Regular Research Grants