Antimicrobial effects of photodynamic therapy with Fotoenticine on Streptococcus mutans isolated from dental caries

Author(s)

Terra-Garcia, Maira ^[1] ; de Souza, Cheyenne Marcal ^[1] ; Ferreira Goncalves, Nathalia Maria ^[1] ; Correia Pereira, Andre Henrique ^[2] ; de Barros, Patricia Pimentel ^[1] ; Borges, Alessandra Buhler ^[3] ; Miyakawa, Walter ^[4] ; Strixino, Juliana Ferreira ^[2] ; Junqueira, Juliana Campos ^[1]

Affiliation

^[1] Sao Paulo State Univ, Inst Sci & Technol, Dept Biosci & Oral Diag, UNESP, Av Francisco Jose Longo 777, BR-12245000 Sao Jose Dos Campos, SP - Brazil

^[2] Univ Vale Paraiba UNIVAP, Photobiol Appl Hlth Res & Dev Inst IP&D, Sao Jose Dos Campos, SP - Brazil

^[3] Sao Paulo State Univ UNESP, Inst Sci & Technol, Dept Restorat Dent, Sao Jose Dos Campos, SP - Brazil

^[4] Inst Adv Studies EFO IEAv, Photon Div, Sao Jose Dos Campos, SP - Brazil

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 34, JUN 2021.
• Web of Science Citations: 0

Abstract

Photodynamic therapy (PDT) is a promising strategy to control cariogenic pathogens, such as Streptococcus mutans. Seeking to reach the total bacterial elimination from dental surfaces, novel photosensitizers have been investigated, such as Fotoenticine (FTC) derived from chlorin e6. The objective of this study was to investigate the photodynamic effects of FTC against several clinical strains of S. mutans. Clinical isolates were obtained from patients with active carious lesions, identified by molecular analysis and subjected to PDT using laser irradiation (660 nm and 39.5 J/cm2) in planktonic and biofilm stages. We identified 11 S. mutans strains from cervical, occlusal and proximal caries. PDT mediated by FTC has totally eliminated the S. mutans cells in planktonic growth for all analyzed strains. In biofilms, PDT with FTC reached statistically significant reductions compared with the non-treated control group, at 5.4, 5.5 and 6.5 Log10 (CFU/mL), respectively, for the strains from proximal, occlusal and cervical caries. The scanning electron microscopy evaluations confirmed that PDT mediated by FTC was able to disaggregate and kill the S. mutans cells adhered to enamel surface, suggesting its potential to disinfect the dental tissues. (AU)

• FAPESP's process: 16/09296-8 - Chlorin as a photosensitizer for photodynamic therapy on Streptococcus mutans
• Grantee: Maíra Terra Garcia
• Support Opportunities: Scholarships in Brazil - Master