Antibacterial Activity of Nanoemulsions Based on Essential Oils Compounds Against Species of Xanthomonas that Cause Citrus Canker

Author(s)

Silva, Gulherme da Cruz ^[1] ; de Oliveira Filho, Josemar Golcalves ^[2] ; Morselli Ribeiro, Marilene de Mori ^[3] ; Oliveira de Souza, Clovis Wesley ^[4] ; Ferreira, Marcos David ^[5]

Affiliation

^[1] Univ Fed Sao Carlos, Dept Biotechnol, Sao Carlos - Brazil

^[2] Sao Paulo State Univ, UNESP, Sch Pharmaceut Sci, Araraquara, SP - Brazil

^[3] Tanquim Ind & Comercio Ltda, Laranjal Paulista - Brazil

^[4] Univ Fed Sao Carlos, Dept Morphol & Patol, Sao Carlos - Brazil

^[5] Embrapa Instrumentacao, Brazilian Agr Res Corp, Sao Carlos - Brazil

• Document type: Journal article
• Source: BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY; v. 12, n. 2, p. 1835-1846, APR 15 2022.
• Web of Science Citations: 0

Abstract

Citrus canker is a bacterial disease that affects citrus crops. caused by microorganisms of the genus Xonfhomonas responsible for great damage to citrus production. In this work the antimicrobial effect of nanoemulsions based on the major compounds of essential oils (EOs) eugenol thymol geraniol and menthone were investigated against two strains of Xanthomonas that cause citrus canker (Xac and XauB). Nanoemulsions ere produced for each compound using two different stirring speeds (7,000 and 12,000 rpm). All nanoemulsions underwent analysis to characterize particle sizes and stability. Thymol nanoemulsions had the smallest particle sizes (50.8 to 73.9 nm) and the highest stability and also showed the ability to inhibit the development of both strains of bacteria. with minimum inhibitory concentrations (MIC) of 0.03% (v/v). along with eugenol (0.03%, (v/v) for Xac and (0.02% (v/v) for XauB) and geraniol nanoemulsions (0.06% (v/v) for both bacteria), Thymol was the compound he highest minimum bactericidal concentrations (MBC). with values of 0.03% (v/v) for both bacterial strains. Eugenol showed MBC only against XauB. at a concentration of 0,0.03%) (v/v). Geraniol did not show bactericidal activity suggesting a bacteriostatic action of this compound with the tested microorganisms. In general. the antimicrobial activity of the nanoemulsions as increased with increased agitation speed. particle reduction and greater stability. Therefore nanoemulsification can be an alternative to applying the antimicrobial activity of natural compounds in the control of citrus canker. (AU)

• FAPESP's process: 18/24612-9 - Application of nanoemulsions with essential oils as natural coatings for application in post-harvesting strawberry conservation
• Grantee: Josemar Gonçalves de Oliveira Filho
• Support Opportunities: Scholarships in Brazil - Doctorate