Simultaneous separation and electroanalysis in a single polydimethylsiloxane-based platform

Author(s)

Maximiano, Elizabete M. ^[1] ; Goncalves, Daniel A. ^[1] ; Martins, Caue A. ^[2] ; Angnes, Lucio ^[3] ; Gomes, Roberto S. ^[4] ; Trindade, Magno A. G. ^{[1, 5]}

Affiliation

^[1] Fundacao Univ Fed Grande Dourados, Fac Ciencias Exatas & Tecnol, Rodovia Dourados Itahum, Km 12, BR-79804970 Dourados, MS - Brazil

^[2] Univ Fed Mato Grosso do Sul, Inst Phys, BR-79070900 Campo Grande, MS - Brazil

^[3] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, Av Prof Lineu Prestes 748, BR-05508000 Sao Paulo, SP - Brazil

^[4] North Dakota State Univ, Dept Pharmaceut Sci, Fargo, ND - USA

^[5] UNESP, Inst Chem, Toxicol Evaluat & Removal Micropollutants & Radio, Natl Inst Alternat Technol Detect, BR-14800900 Araraquara, SP - Brazil

• Document type: Journal article
• Source: Talanta; v. 233, OCT 1 2021.
• Web of Science Citations: 0

Abstract

Channel-based microfluidic devices integrating the separation step and detection system are key factors to expand microanalysis application. However, these devices still depend on macroscale external equipment for pre-treatment of the sample, separation, or detection. The integration of all steps in only one stage is critical to improving feasibility. Herein, we use a low-cost protocol to solve part of the challenge by designing a dual-mode system onto single polydimethylsiloxane (PDMS)-based platform - overall dimensions of 65 mm length x 20 mm width x 14 mm height and the inner diameter of 297 +/- 10 mu m height x 605 +/- 19 mu m width - for column-free separation and simultaneous detection. As a proof-of-concept, we used this all-in-one PDMS platform to separate - without the packet-based phase - and determine salicylic acid (SA) and caffeine (CAF) with a detection limit of 0.20 and 0.18 mu mol L-1 and quantification limit of 0.70 and 0.60 mu mol L-1 for SA and CAF, respectively. We separated the mixture using forced convection into a chemically treated microchannel while detecting the analytes in amperometric mode. Here, we report new insights into how integrating analytes separation and further electroanalysis into a single miniaturized device. (AU)

• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants