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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of biostimulation and bioaugmentation on hydrocarbon degradation and detoxification of diesel-contaminated soil: a microcosm study

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Author(s):
Giovanella, Patricia [1] ; Duarte, Lidia de Azevedo [1] ; Kita, Daniela Mayumi [1] ; de Oliveira, Valeria Maia [2] ; Sette, Lara Duraes [1]
Total Authors: 5
Affiliation:
[1] Sao Paulo State Univ UNESP, Inst Biosci, Dept Gen & Appl Biol, Lab Environm & Ind Mycol, BR-01049010 Rio Claro, SP - Brazil
[2] State Univ Campinas UNICAMP, Res Ctr Chem Biol & Agr, Microbial Resources Div, BR-13083970 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: JOURNAL OF MICROBIOLOGY; v. 59, n. 7, p. 634-643, JUL 2021.
Web of Science Citations: 0
Abstract

Soil contamination with diesel oil is quite common during processes of transport and storage. Bioremediation is considered a safe, economical, and environmentally friendly approach for contaminated soil treatment. In this context, studies using hydrocarbon bioremediation have focused on total petroleum hydrocarbon (TPH) analysis to assess process effectiveness, while ecotoxicity has been neglected. Thus, this study aimed to select a microbial consortium capable of detoxifying diesel oil and apply this consortium to the bioremediation of soil contaminated with this environmental pollutant through different bioremediation approaches. Gas chromatography (GC-FID) was used to analyze diesel oil degradation, while ecotoxicological bioassays with the bioindicators Artemia sp., Aliivibrio fischeri (Microtox), and Cucumis sativus were used to assess detoxification. After 90 days of bioremediation, we found that the biostimulation and biostimulation/bioaugmentation approaches showed higher rates of diesel oil degradation in relation to natural attenuation (41.9 and 26.7%, respectively). Phytotoxicity increased in the biostimulation and biostimulation/bioaugmentation treatments during the degradation process, whereas in the Microtox test, the toxicity was the same in these treatments as that in the natural attenuation treatment. In both the phytotoxicity and Microtox tests, bioaugmentation treatment showed lower toxicity. However, compared with natural attenuation, this approach did not show satisfactory hydrocarbon degradation. Based on the microcosm experiments results, we conclude that a broader analysis of the success of bioremediation requires the performance of toxicity bioassays. (AU)

FAPESP's process: 13/19486-0 - Marine and Antarctic biotechnology: microbial enzymes and their applications
Grantee:Lara Durães Sette
Support Opportunities: Regular Research Grants
FAPESP's process: 16/17488-4 - Bioremediation of contaminated soils with diesel oil: structural and functional analysis of the microbial community
Grantee:Patrícia Giovanella
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/13205-2 - Detoxification and degradation of petroleum and diesel oil by microbial marine consortia
Grantee:Lidia de Azevedo Duarte
Support Opportunities: Scholarships in Brazil - Master