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Bioremediation of contaminated soils with diesel oil: structural and functional analysis of the microbial community

Grant number: 16/17488-4
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): April 01, 2017
Effective date (End): November 27, 2020
Field of knowledge:Biological Sciences - Microbiology
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Lara Durães Sette
Grantee:Patrícia Giovanella
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Associated scholarship(s):19/11823-4 - Metabolomics of diesel oil contaminated soil after bioremediation, BE.EP.PD


The diesel oil is the main fuel in the Brazilian energetic matrix, being the petroleum derivative most commercialized in Brazil. This fuel is a complex mix of hydrocarbons and, due to its recalcitrance and frequent spill accidents, it is worldwide recognized as a threat to aquatic and terrestrial ecosystems. Several techniques are available to the remediation of sites contaminated with hydrocarbons. However, the bioremediation is considered a promising alternative since it is more sustainable, efficient and cost-effective when compared to other remediation technologies. In the present study, distinct strategies will be used for detoxification/degradation of soils contaminated with diesel oil (natural attenuation, bioaugmentation, and biostimulation), in order to select the best conditions of bioremediation for this pollutant. It will be evaluated a microbial consortium composed by marine fungi, bacteria from oil reservoir (off-shore), and yeast from Antartic, which was able to detoxify efficiently the diesel oil after 21 days of submerged cultivation in previous studies. The diesel oil degradation in the distinct treatments will be monitored through gas chromatography, while the detoxification will be performed by acute toxicity analysis with Microtox (Vibrio fisheri) and the microcrustacean Artemia sp. This project aims to elucidate the complex microbial mechanisms involved in the diesel oil detoxification/degradation (in the selected treatment) by metatranscriptome, as well as monitor the dynamics of the microbial community during the detoxification/degradation process using genetic fingerprinting (PCD-DGGE). The access to the taxonomic profile of the microorganisms involved in the detoxification/degradation process will be carry out by cloning and sequencing. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GIOVANELLA, PATRICIA; VIEIRA, GABRIELA A. L.; RAMOS OTERO, IGOR V.; PELLIZZER, ELISA PAIS; FONTES, BRUNO DE JESUS; SETTE, LARA D. Metal and organic pollutants bioremediation by extremophile microorganisms. JOURNAL OF HAZARDOUS MATERIALS, v. 382, JAN 15 2020. Web of Science Citations: 1.

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