Bioremediation of contaminated soils with diesel oil: structural and functional analysis of the microbial community

• Grant number: 16/17488-4
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: April 01, 2017
• End date: November 27, 2020
• Field of knowledge: Biological Sciences - Microbiology
• Agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Lara Durães Sette
• Grantee: Patrícia Giovanella
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Associated scholarship(s): 19/11823-4 - Metabolomics of diesel oil contaminated soil after bioremediation, BE.EP.PD

Abstract

The diesel oil is the main fuel in the Brazilian energetic matrix, being the petroleum derivative most commercialized in Brazil. This fuel is a complex mix of hydrocarbons and, due to its recalcitrance and frequent spill accidents, it is worldwide recognized as a threat to aquatic and terrestrial ecosystems. Several techniques are available to the remediation of sites contaminated with hydrocarbons. However, the bioremediation is considered a promising alternative since it is more sustainable, efficient and cost-effective when compared to other remediation technologies. In the present study, distinct strategies will be used for detoxification/degradation of soils contaminated with diesel oil (natural attenuation, bioaugmentation, and biostimulation), in order to select the best conditions of bioremediation for this pollutant. It will be evaluated a microbial consortium composed by marine fungi, bacteria from oil reservoir (off-shore), and yeast from Antartic, which was able to detoxify efficiently the diesel oil after 21 days of submerged cultivation in previous studies. The diesel oil degradation in the distinct treatments will be monitored through gas chromatography, while the detoxification will be performed by acute toxicity analysis with Microtox (Vibrio fisheri) and the microcrustacean Artemia sp. This project aims to elucidate the complex microbial mechanisms involved in the diesel oil detoxification/degradation (in the selected treatment) by metatranscriptome, as well as monitor the dynamics of the microbial community during the detoxification/degradation process using genetic fingerprinting (PCD-DGGE). The access to the taxonomic profile of the microorganisms involved in the detoxification/degradation process will be carry out by cloning and sequencing. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DANIELA M. KITA; PATRICIA GIOVANELLA; THAÍS T. YOSHINAGA; ELISA P. PELLIZZER; LARA D. SETTE. Antarctic fungi applied to textile dye bioremediation. Anais da Academia Brasileira de Ciências, v. 94, 2022-02-11. (18/12098-9, 16/17488-4, 19/01632-7)

CABRAL, LUCELIA; GIOVANELLA, PATRICIA; PELLIZZER, ELISA PAIS; TERAMOTO, ELIAS HIDEO; KIANG, CHANG HUNG; SETTE, LARA DURAES. Microbial communities in petroleum-contaminated sites: Structure and metabolisms. Chemosphere, v. 286, n. 2, JAN 2022. (19/11823-4, 16/17488-4)

GIOVANELLA, PATRICIA; TAKETANI, RODRIGO GOUVEA; GIL-SOLSONA, RUBEN; SALDANHA, LUIZ LEONARDO; NARANJO, SAMANTHA BEATRIZ ESPARZA; SANCHO, JUAN V.; PORTOLES, TANIA; ANDREOTE, FERNANDO DINI; RODRIGUEZ-MOZAZ, SARA; BARCELO, DAMIA; et al. A comprehensive study on diesel oil bioremediation under microcosm conditions using a combined microbiological, enzymatic, mass spectrometry, and metabarcoding approach. Environmental Science and Pollution Research, v. N/A, p. 17-pg., 2023-08-30. (16/17488-4, 19/11823-4, 18/10734-5)

GIOVANELLA, PATRICIA; DUARTE, LIDIA DE AZEVEDO; KITA, DANIELA MAYUMI; DE OLIVEIRA, VALERIA MAIA; SETTE, LARA DURAES. Effect of biostimulation and bioaugmentation on hydrocarbon degradation and detoxification of diesel-contaminated soil: a microcosm study. JOURNAL OF MICROBIOLOGY, v. 59, n. 7, p. 634-643, JUL 2021. (13/19486-0, 16/17488-4, 14/13205-2)

GIOVANELLA, PATRICIA; VIEIRA, GABRIELA A. L.; RAMOS OTERO, IGOR V.; PELLIZZER, ELISA PAIS; FONTES, BRUNO DE JESUS; SETTE, LARA D.. Metal and organic pollutants bioremediation by extremophile microorganisms. JOURNAL OF HAZARDOUS MATERIALS, v. 382, JAN 15 2020. (16/17488-4, 18/12098-9, 16/07957-7, 12/12622-3)