Maternal Apical Periodontitis Increases Insulin Resistance and Modulates the Antioxidant Defense System in the Gastrocnemius Muscle of Adult Offspring

Author(s)

Saori Tsosura, Thais Veronica ^{[1, 2]} ; dos Santos, Rodrigo Martins ^{[1, 2]} ; Chaves Neto, Antonio Hernandes ^{[1, 2]} ; Chiba, Fernando Yamamoto ^[3] ; Nascimento Carnevali, Ana Carolina ^[1] ; de Lima Coutinho Mattera, Maria Sara ^[1] ; Belardi, Bianca Elvira ^{[1, 2]} ; Angelo Cintra, Luciano Tavares ^[3] ; da Silva Machado, Nathalia Evelyn ^[3] ; Matsushita, Doris Hissako ^{[1, 2]}

Affiliation

^[1] Sao Paulo State Univ, UNESP, Sch Dent, Dept Basic Sci, Aracatuba - Brazil

^[2] Sao Paulo State Univ, UNESP, Soc Brasileira Fisiol SBFis, Sch Dent, Programa Posgrad Multicentr Ciencias Fisiol PPGMC, Aracatuba - Brazil

^[3] Sao Paulo State Univ, UNESP, Sch Dent, Dept Prevent & Restorat Dent, Aracatuba - Brazil

• Document type: Journal article
• Source: JOURNAL OF ENDODONTICS; v. 47, n. 7, p. 1126-1131, JUL 2021.
• Web of Science Citations: 1

Abstract

Introduction: Maternal apical periodontitis (AP) is associated with insulin resistance (IR) in adult offspring. Oxidative stress has been linked to IR. This study investigated insulin sensitivity (IS) and oxidative stress in the gastrocnemius muscle (GM) of adult offspring of rats with AP. Methods: Fifteen female Wistar rats were distributed into a control group, a group with 1 tooth with AP, and a group with 4 teeth with AP. Thirty days after AP induction, female rats were mated with healthy male rats. When male offspring reached 75 days of age, glycemia, insulinemia, and IS were determined. In the GM, the oxidative damage products (thiobarbituric acid reactive substances and carbonyl protein) and activities of enzymatic (superoxide dismutase, catalase, and glutathione peroxidase) and nonenzymatic (glutathione and total antioxidant capacity) antioxidants were quantified. Analysis of variance was performed followed by the Tukey post hoc test (P , .05). Results: Maternal AP was associated with decreased IS and changes in antioxidant activities (reduced superoxide dismutase and increased catalase, glutathione peroxidase, and glutathione) and decreased thiobarbituric acid reactive substance concentration in the GM of their adult offspring. However, maternal AP does not appear to affect glycemia, carbonyl protein concentration, and the nonenzymatic total antioxidant capacity in the GM of this offspring. Conclusions: Maternal AP modulates the antioxidant defense system in the GM of their adult offspring, attenuating lipid peroxidation in this tissue. This reflects part of an adaptive response of the offspring to the stimulation of the maternal chronic oral inflammatory process in which the organism acts by decreasing oxidative tissue damage in the postnatal stage. The present study improves knowledge about the impact of maternal oral inflammation on healthy offspring. (J Endod 2021;47:1126-1131.) (AU)

• FAPESP's process: 20/08706-3 - Evaluation of protein content and DNA methylation of GLUT4 in the gastrocnemius muscle of adult rats, offspring of rats with apical periodontitis
• Grantee: Ana Carolina Nascimento Carnevali
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 19/04182-2 - Assessment of the final steps of insulin signaling, inflammatory pathway, oxidative stress, epigenetic mechanism by DNA methylation and GLUT4 expression in the gastrocnemius muscle of adult rats, offspring of rats with Apical Periodontitis
• Grantee: Thaís Verônica Saori Tsosura
• Support Opportunities: Scholarships in Brazil - Doctorate