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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Enhancement of Clostridium butyricum hydrogen production by iron and nickel nanoparticles: Effects on hydA expression

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Author(s):
Moura, A. G. L. [1] ; Rabelo, C. A. B. S. [1] ; Okino, C. H. [2] ; Maintinguer, I, S. ; Silva, E. L. [3] ; Varesche, M. B. A. [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo EESC USP, Engn Sch Sao Carlos, Lab Biol Proc, Dept Hydraul & Sanitat, Sao Carlos, SP - Brazil
[2] Embrapa Southeast Livestock, Farm Canchim, Washington Luiz Km 234, Sao Carlos, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Chem Engn, Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY; v. 45, n. 53, p. 28447-28461, OCT 30 2020.
Web of Science Citations: 4
Abstract

In this study, an optimized medium for Clostridium butyricum (CCT7470) was obtained from a Plackett-Burman assay. It was evidenced that glucose (5 g/L), starch (1 g/L) and three nitrogen and vitamin sources (5 g meat extract/L; 5 g peptone/L and 1.5 g yeast extract/L) improved hydrogen (H-2) production. The addition of nanoparticles (NPs) improved the fermentation process, and maximum H-2 production (434 +/- 13 mL) was achieved with 30 mg Fe-0/L and 20 mg Ni-0/L. The metabolic pathway shifted from acetic and butyric to valeric acid production with the addition of NPs. In reactors with NPs, a longer permanence of hydrogenase was observed with hydA quantification by real-time polymerase chain reaction (qPCR). However, the expression of new enzymes, assessed by reverse transcription followed by qPCR (RT-qPCR), was higher in control assay than that in the NPs one. (C) 2020 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 18/26470-7 - Influence of sugarcane bagasse saccharification by laccase for fermentative production of biofuels
Grantee:Camila Abreu Borges da Silva Rabelo
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 17/23008-8 - Assessment of Iron and Nickel nanoparticles influence on batch and continuous reactors fermentative hydrogen production
Grantee:Alana Gandra Lima de Moura
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 15/06246-7 - Biorefinery concept applied to biological wastewater treatment plants: environmental pollution control coupled with material and energy recovery
Grantee:Marcelo Zaiat
Support Opportunities: Research Projects - Thematic Grants