Excessive caffeine intake increases bone resorption associated with periapical periodontitis in rats

Author(s)

Dal-Fabbro, Renan ^[1] ; Cosme-Silva, Leopoldo ^[2] ; Capalbo, Leticia Cabrera ^[1] ; Chaves-Neto, Antonio Hernandes ^[3] ; Ervolino, Edilson ^[3] ; Cintra, Luciano Tavares Angelo ^[1] ; Gomes-Filho, Joao Eduardo ^[1]

Affiliation

^[1] Dept Prevent & Restorat Dent, Aracatuba - Brazil

^[2] Fed Univ Alagoas UFAL, Sch Dent, Dept Restorat Dent & Endodont, Alagoas - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Dent, Dept Basic Sci, Aracatuba - Brazil

• Document type: Journal article
• Source: International Endodontic Journal; v. 54, n. 10 JUL 2021.
• Web of Science Citations: 1

Abstract

Aim: To evaluate the effect of excessive caffeine intake on the inflammation/resorption processes associated with periapical periodontitis (PP) in rats. Methodology: Sixteen Wistar rats were used. Periapical periodontitis was induced in the four first molars in each animal. The animals were arranged into two groups: control (C)-rats with periapical periodontitis; and caffeine (CAF)-rats with periapical periodontitis under caffeine administration protocol. The CAF animals received 10 mg/100 g of body weight/day of caffeine via gavage starting fifteen days before PP induction and continuing for thirty more days until euthanasia. On the 30th day, the animals were euthanized and the jaws removed for microcomputed tomography, histological and immunohistochemical analysis for RANKL, OPG, TRAP, IL-10, TNF-alpha and IL-1 beta. The Mann-Whitney test was performed for nonparametric data, and Student's t test was performed for parametric data, using p < .05. Results: There was no significant difference in the weight change between the groups. The median score of the inflammatory process was significantly greater in the CAF group (3) compared with the C group (2), p = .0256. Bone resorption was greater in the group consuming caffeine (1.08 +/- 0.15 mm(3)) compared with the C group (0.88 +/- 0.10 mm(3)), p = .0346. The immunolabelling for RANKL, TRAP and IL-1 beta was significantly higher in the CAF group when compared to the control, p < .05. No differences were found for the OPG, IL-10 and TNF-alpha immunolabelling. Conclusion: Excessive caffeine exposure via gavage in rats was able to exacerbate the volume of periapical bone destruction, and the inflammatory pattern deriving from periapical periodontitis altering the expression of RANKL, IL-1 beta and TRAP. (AU)

• FAPESP's process: 17/27219-3 - Effect of red wine consumption on the induced Apical Periodontitis development in rats
• Grantee: Renan Dal Fabbro
• Support Opportunities: Scholarships in Brazil - Doctorate