Burkholderia sacchari (synonym Paraburkholderia sacchari): An industrial and versatile bacterial chassis for sustainable biosynthesis of polyhydroxyalkanoates and other bioproducts

Author(s)

Oliveira-Filho, Edmar Ramos ^[1] ; Gomez, Jose Gregorio Cabrera ^[1] ; Taciro, Marilda Keico ^[1] ; Silva, Luiziana Ferreira ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

• Document type: Journal article
• Source: Bioresource Technology; v. 337, OCT 2021.
• Web of Science Citations: 0

Abstract

This is the first review presenting and discussing Burkholderia sacchari as a bacterial chassis. B. sacchari is a distinguished polyhydroxyalkanoates producer strain, with low biological risk, reaching high biopolymer yields from sucrose (0.29 g/g), and xylose (0.38 g/g). It has great potential for integration into a biorefinery using residues from biomass, achieving 146 g/L cell dry weight containing 72% polyhydroxyalkanoates. Xylitol (about 70 g/L) and xylonic acid {[}about 390 g/L, productivity 7.7 g/(L.h)] are produced by the wild-type B. sacchari. Recombinants were constructed to allow the production and monomer composition control of diverse tailormade polyhydroxyalkanoates, and some applications have been tested. 3-hydroxyvalerate and 3-hydroxyhexanoate yields from substrate reached 80% and 50%, respectively. The genome-scale reconstruction of its metabolic network, associated with the improvement of tools for genetic modification, and metabolic fluxes understanding by future research, will consolidate its potential as a bioproduction chassis. (AU)

• FAPESP's process: 16/00842-0 - Enhancing pentose and co-substrate bacterial uptake for the production of biopolymers from biomass: strain improvement, bioprocess optimization and application tests
• Grantee: Luiziana Ferreira da Silva
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants