| Full text | |
| Author(s): Show less - |
Santana, Fernanda P. R.
[1]
;
Ricardo-da-Silva, Fernanda Y.
[2]
;
Fantozzi, Evelyn T.
[3]
;
Pinheiro, Nathalia M.
[4, 5]
;
Tiberio, Iolanda F. L. C.
[4]
;
Moreira, Luiz Felipe Pinho
[2]
;
Prado, Marco Antonio M.
[6, 7]
;
Prado, Vania F.
[6, 7]
;
Tavares-de-Lima, Wothan
[3]
;
Prado, Carla Maximo
[1, 4, 5]
;
Breithaupt-Faloppa, Ana Cristina
[2]
Total Authors: 11
|
| Affiliation: | [1] Univ Fed Sao Paulo, Dept Biol Sci, Diadema - Brazil
[2] Univ Sao Paulo, Fac Med, Inst Coracao InCor, Lab Cirurgia Cardiovasc & Fisiopatol Circulacao L, Sao Paulo - Brazil
[3] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo - Brazil
[4] Univ Sao Paulo, Sch Med, Dept Internal Med, Sao Paulo - Brazil
[5] Univ Fed Sao Paulo, Dept Biosci, Campus Baixada Santista, Rua Silva Jardim, Santos, SP - Brazil
[6] Univ Western Ontario, Dept Anat & Cell Biol, London, ON - Canada
[7] Univ Western Ontario, Dept Physiol & Pharmacol, Robarts Res Inst, London, ON - Canada
Total Affiliations: 7
|
| Document type: | Journal article |
| Source: | Inflammation; v. 44, n. 4, p. 1553-1564, AUG 2021. |
| Web of Science Citations: | 1 |
| Abstract | |
Acute lung injury induced by intestinal ischemia/reperfusion (I/R) is a relevant clinical condition. Acetylcholine (ACh) and the alpha 7 nicotinic ACh receptor (nAChR alpha-7) are involved in the control of inflammation. Mice with reduced levels of the vesicular ACh transporter (VAChT), a protein responsible for controlling ACh release, were used to test the involvement of cholinergic signaling in lung inflammation due to intestinal I/R. Female mice with reduced levels of VAChT (VAChT-KDHOM) or wild-type littermate controls (WT) were submitted to intestinal I/R followed by 2 h of reperfusion. Mortality, vascular permeability, and recruitment of inflammatory cells into the lung were investigated. Parts of mice were submitted to ovariectomy (OVx) to study the effect of sex hormones or treated with PNU-282,987 (nAChR alpha-7 agonist). A total of 43.4% of VAChT-KDHOM-I/R mice died in the reperfusion period compared to 5.2% of WT I/R mice. The I/R increased lung inflammation in both genotypes. In VAChT-KDHOM mice, I/R increased vascular permeability and decreased the release of cytokines in the lung compared to WT I/R mice. Ovariectomy reduced lung inflammation and permeability compared to non-OVx, but it did not avoid mortality in VAChT-KDHOM-I/R mice. PNU treatment reduced lung permeability, increased the release of proinflammatory cytokines and the myeloperoxidase activity in the lungs, and prevented the increased mortality observed in VAChT-KDHOM mice. Cholinergic signaling is an important component of the lung protector response against intestinal I/R injury. Decreased cholinergic signaling seems to increase pulmonary edema and dysfunctional cytokine release that increased mortality, which can be prevented by increasing activation of nAChR alpha-7. (AU) | |
| FAPESP's process: | 14/25689-4 - Effects of cholinergic system in acute and chronic pulmonary inflammation |
| Grantee: | Carla Máximo Prado |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 18/06088-0 - Effects of eugenol and dehidrodieugenol isolated from Nectandra leucantha (Lauraceae) treatments in experimental models of acute and chronic lung disease |
| Grantee: | Carla Máximo Prado |
| Support Opportunities: | Regular Research Grants |