Odontogenesis-related candidate genes involved in variations of permanent teeth size

Author(s)

Gerber, Jennifer Tsi ^[1] ; dos Santos, Katheleen Miranda ^[1] ; Brum, Bruna Karas ^[1] ; Petinati, Maria Fernanda Pivetta ^[1] ; Meger, Michelle Nascimento ^[1] ; da Costa, Delson Joao ^[2] ; Elsalanty, Mohammed ^[3] ; Kuchler, Erika Calvano ^[4] ; Scariot, Rafaela ^[2]

Affiliation

^[1] Positivo Univ, Sch Hlth Sci, 5300 Prof Pedro Viriato Parigot de Souza St, BR-81280330 Curitiba, PR - Brazil

^[2] Univ Fed Parana, Sch Dent, Dept Stomatol, 632 Prefeito Lothario Meissner Ave, BR-80210170 Curitiba, PR - Brazil

^[3] Western Univ, Dept Med & Anat Sci, Coll Ostheopath Med Pacific, 615 E 3rd St, Pomona, CA 91766 - USA

^[4] Univ Sao Paulo, Dept Pediat Dent, Sch Dent Ribeirao Preto, Ave Cafe S-N, Campus USP, BR-14040904 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: CLINICAL ORAL INVESTIGATIONS; v. 25, n. 7, p. 4481-4494, JUL 2021.
• Web of Science Citations: 0

Abstract

Objective The aim of the study was to evaluate the association between genetic polymorphisms in RUNX2, BMP4, BMP2, TGF beta 1, EGF, and SMAD6 and variations in permanent tooth size (TS). Materials and methods This cross-sectional study evaluated 110 individuals' dental casts to determine the maximum tooth crown size of all fully erupted permanent teeth (third molars were excluded) in the mesiodistal (MD) and buccolingual (BL) dimensions. Genomic DNA was obtained from the epithelial cells of the oral mucosa to evaluate the genetic polymorphisms in RUNX2 (rs59983488 and rs1200425), BMP4 (rs17563), BMP2 (rs235768 and rs1005464), TGF beta 1 (rs1800470), EGF (rs4444903), and SMAD6 (rs2119261 and rs3934908) through real-time PCR. The data were submitted to statistical analysis with a significance level of 0.05. Results The genetic polymorphisms rs59983488, rs1200425, rs17563, rs235768, rs1005464, rs1800470, and rs4444903 were associated with MD and BL TS of the upper and lower arches (p < 0.05). The polymorphism rs2119261 was associated with variation in TS only in the upper arch (p < 0.05). The rs3934908 was not associated with any TS measurement (p > 0.05). Conclusions In summary, this study reports novel associations between variation in permanent TS and genetic polymorphisms in RUNX2, BMP4, BMP2, TGF beta 1, EGF, and SMAD6 indicating a possible role of these genes in dental morphology. (AU)

• FAPESP's process: 15/06866-5 - Evaluation of the role of estrogen in dentofacial development
• Grantee: Erika Calvano Kuchler
• Support Opportunities: Research Grants - Young Investigators Grants