Involvement of substance P, osteopontin and satellite glial cells on photobiomodulation-induced antinociceptive effect in an experimental model of dentin hypersensitivity

Author(s)

de Oliveira, Victhor Teixeira ^[1] ; Ferrara-Jr, Joao Ignacio ; Matielo, Heloisa Alonso ^[2] ; Alves, Adilson da Silv ^[3] ; Britto, Luiz Roberto ^[3] ; Correa Aranha, Ana Cecilia ^[4] ; Dale, Camila Squarzoni ^[2]

Affiliation

^[1] Univ Sao Paulo, Dept Anat, Lab Neuromodulat Expt Pain, Av Lineu Prestes 2415, ICH 3, BR-05508000 Sao Paulo - Brazil

^[2] Ferrara-Jr, Jr., Joao Ignacio, Univ Sao Paulo, Dept Anat, Lab Neuromodulat Expt Pain, Av Lineu Prestes 2415, ICH 3, BR-05508000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Physiol, Lab Cellular Neurobiol, Av Lineu Prestes 1524, ICH 1, BR-05508000 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Special Lab Lasers Dent LELO, Av Prof Lineu Prestes 2227, BR-05508000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 36, n. 6, p. 1297-1305, AUG 2021.
• Web of Science Citations: 0

Abstract

The aim of this work was to investigate the involvement of substance P (SP), osteopontin (OPN), and satellite glial cells (SGC) on photobiomodulation-induced (PBM) antinociceptive effect in an experimental model of dentin hypersensitivity (DH). Rats ingested isotonic drink (ID, pH 2.87) for 45 consecutive days and after this period received PBM irradiation at lambda 660 nm or lambda 808 nm (1 J, 3.5 J/cm(2), 100 mW, 10 s, 0.028 cm(2), continuous wave, 3 consecutive daily sessions), and were evaluated for nociceptive behavior 24, 48, 72 h, and 14 days after laser treatments. ID ingestion induced an increase on thermal sensitivity of DH characteristics in rats that was completely reversed by PBM treatment at both 660 and 808 nm. Immunohistochemical analysis revealed increased SP expression at both dentin-pulp complex (DPC) and trigeminal ganglia (TG) of DH-rats which did not occur in PBM groups by PBM treatment. Also, the increase of glial fibrillary acidic protein (GFAP) observed in the TG of DH-rats was also reversed by PBM treatment. Finally, PBM at both 660 and 808 nm increased OPN expression in the dentin-pulp complex of DH-rats after 14 days of PBM treatment. All in all, this data demonstrates that PBM reverses nociception in a DH experimental model by inhibiting neurogenic inflammation and inducing a regenerative response. (AU)

• FAPESP's process: 18/14560-1 - "Neuropathic pain prevalence in diabetic patients, validation of pain screening instruments, correlation with cutaneous neuropathology, TNF alpha-gene polymorphism, tissular cicatricial process and effect of photobiomodulation therapy"
• Grantee: Camila Squarzoni Dale
• Support Opportunities: Regular Research Grants